Literature DB >> 6048803

Enzymes and cancer. Preparation and some properties of guanase from rabbit liver.

R Currie, F Bergel, R C Bray.   

Abstract

1. Guanase has been purified 200-fold in 20% yield from the supernatant fraction of rabbit-liver homogenates, by using ammonium sulphate fractionation, calcium phosphate-gel adsorption and chromatography on DEAE-cellulose and Sephadex G-200. 2. K(m) with guanine as substrate at the optimum pH of 7.7 was found to be 1.05x10(-5)m. Q(10) was 1.4 between 23 degrees and 48 degrees . 3. Substrate activity and pH optima of compounds related to guanine have been studied. 8-Azaguanine, 1-methylguanine, thioguanine and 1-methylthioguanine are all substrates.

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Year:  1967        PMID： 6048803      PMCID： PMC1270630          DOI： 10.1042/bj1040634

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

1.  DIRECT STUDIES ON THE ELECTRON TRANSFER SEQUENCE IN XANTHINE OXIDASE BY ELECTRON PARAMAGNETIC RESONANCE SPECTROSCOPY. I. TECHNIQUES AND DESCRIPTION OF SPECTRA.

Authors:  G PALMER; R C BRAY; H BEINERT
Journal:  J Biol Chem       Date:  1964-08       Impact factor: 5.157

2.  Guanine degradation by Clostridium acidiurici. I. Evidence for the presence of guanase.

Authors:  J RAKOSKY; J V BECK
Journal:  J Bacteriol       Date:  1955-05       Impact factor: 3.490

3.  Guanine degradation by Clostridium acidiurici. II. Isolation and characterization of guanase.

Authors:  J RAKOSKY; L N ZIMMERMAN; J V BECK
Journal:  J Bacteriol       Date:  1955-05       Impact factor: 3.490

4.  [Direct determination of ammonium in blood and tissue extracts by means of the phenol by chlorite reaction].

Authors:  K KONITZER; S VOIGT
Journal:  Clin Chim Acta       Date:  1963-01       Impact factor: 3.786

5.  Regulatory mechanisms in carbohydrate metabolism. III. Limiting factors in glycolysis of ascites tumor cells.

Authors:  R WU; E RACKER
Journal:  J Biol Chem       Date:  1959-05       Impact factor: 5.157

6.  Protein chromatography on calcium phosphate columns.

Authors:  S HJERTEN; O LEVIN; A TISELIUS
Journal:  Arch Biochem Biophys       Date:  1956-11       Impact factor: 4.013

7.  The Identification of Guanine in Extracts of Girella Nigricans: The Specificity of Guanase.

Authors:  G H Hitchings; E A Falco
Journal:  Proc Natl Acad Sci U S A       Date:  1944-10-15       Impact factor: 11.205

8.  Deamination of 8-azaguanine by guanase.

Authors:  A ROUSH; E R NORRIS
Journal:  Arch Biochem       Date:  1950-11

9.  Lingcod muscle guanine deaminase.

Authors:  J E Roy
Journal:  Can J Biochem       Date:  1966-08

10.  Estimation of the molecular weights of proteins by Sephadex gel-filtration.

Authors:  P Andrews
Journal:  Biochem J       Date:  1964-05       Impact factor: 3.766
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