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Literature DB >> 6039374

Absolute sensitivity of electron microscope radioautography.

Abstract

A calibration method is described for measuring absolute radioautographic sensitivities under various experimental conditions. Sensitivities to (3)H and (35)S radiation, i.e. ratio of developed grains to radioactive decays in the specimen, were determined with Ilford L4 and Kodak NTE emulsions. The highest values obtained in monolayers of emulsion were (1/8) for (3)H and 1/21 for (35)S. The influence of various experimental parameters on sensitivity is described, and the possibilities for quantitative electron microscope radioautography are discussed.

Entities: Chemical Gene

Mesh：

Substances：
Sulfur Isotopes
Tritium

Year: 1967 PMID： 6039374 PMCID： PMC2108360 DOI： 10.1083/jcb.33.2.299

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

6 in total

1. AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE; A QUANTITATIVE EVALUATION.

Authors: L BACHMANN; M M SALPETER
Journal: Lab Invest Date: 1965-06 Impact factor: 5.662

2. High-resolution autoradiography. I. Methods.

Authors: L G CARO; R P VAN TUBERGEN; J A KOLB
Journal: J Cell Biol Date: 1962-11 Impact factor: 10.539

3. Tritium and Phosphorus-32 in High-Resolution Autoradiography.

Authors: L G Caro; M Schnös
Journal: Science Date: 1965-07-02 Impact factor: 47.728

4. Electron microscope radioautography as a quantitative tool in enzyme cytochemistry. I. The distribution of acetylcholinesterase at motor end plates of a vertebrate twitch muscle.

Authors: M M Salpeter
Journal: J Cell Biol Date: 1967-02 Impact factor: 10.539

5. AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE. A PROCEDURE FOR IMPROVING RESOLUTION, SENSITIVITY, AND CONTRAST.

Authors: M M SALPETER; L BACHMANN
Journal: J Cell Biol Date: 1964-08 Impact factor: 10.539

6. High-resolution autoradiogaphy. II. The problem of resolution.

Authors: L G CARO
Journal: J Cell Biol Date: 1962-11 Impact factor: 10.539

6 in total

15 in total

Review 1. Non-isotopic electron microscope in situ hybridization for studying the functional sub-compartmentalization of the cell nucleus.

Authors: F Puvion-Dutilleul; E Puvion
Journal: Histochem Cell Biol Date: 1996-07 Impact factor: 4.304

2. The autoradiographical assoc-iation of fast transported material with dense core vesicles in the central nervous system of Anodonta cygnea (l).

Authors: E A Howes; B J McLaughlin; J P Heslop
Journal: Cell Tissue Res Date: 1974 Impact factor: 5.249

8. The distribution of labeled norepinephrine within sympathetic nerve terminals studied with electron microscope radioautography.

Authors: G C Budd; M M Salpeter
Journal: J Cell Biol Date: 1969-04-01 Impact factor: 10.539

9. Uptake and subcellular distribution of [3H]arachidonic acid in murine fibrosarcoma cells measured by electron microscope autoradiography.

Authors: E J Neufeld; P W Majerus; C M Krueger; J E Saffitz
Journal: J Cell Biol Date: 1985-08 Impact factor: 10.539

10. Glutamate uptake by a stimulated insect nerve muscle preparation.

Authors: I R Faeder; M M Salpeter
Journal: J Cell Biol Date: 1970-08 Impact factor: 10.539