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Literature DB >> 60349

Section preparation of human marrow for light microscopy.

J T Dancey, K A Deubelbeiss, L A Harker, J Greenaway.

Abstract

Preparation of human marrow sections has been studied systematically in order to facilitate accurate identification of marrow cells. Both of the methods developed involve embedding marrow cores in methyl methacrylate. In one, acrolein fixation is followed by staining of deplasticized sections with eosine-y followed by azure II; in the other, neutrophilic forms are identified by their esterase-specific reactivity in marrow fixed with neutral-buffered formalin. These preparations are suitable for quantitative studies of marrow cellularity.

Entities: Chemical Species

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Year: 1976 PMID： 60349 PMCID： PMC476150 DOI： 10.1136/jcp.29.8.704

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

12 in total

9. [Preparative prerequisites in clinical histology of human bone marrow. 1. Methodical studies on embedding in acrylates of lipid-rich tissue material].

Authors: R Burkhardt
Journal: Blut Date: 1966-09

10. The demonstration of anions generated by the action of sodium hypochlorite (NaOCl) on tissue sections, including observations on the unmasking of carbonyl groups.

Authors: C L Ruddell
Journal: Histochemie Date: 1969

3 in total

Section preparation of human marrow for light microscopy.

1. NEEDLE BIOPSY OF BONE AND MARROW; AN EXPERIENCE WITH 1,445 BIOPSIES.

2. A PLASTIC EMBEDDING MEDIUM FOR THIN SECTIONING IN LIGHT MICROSCOPY.

3. An adaptation of the Maximow technique for preparation of sections of hematopoietic tissues.

4. An adaptation of methacrylate embedding for routine histopathologic use.

5. Non-Hodgkin's lymphomas. I. Bone marrow involvement.

6. Clinicopathologic studies of 117 untreated patients subjected to laparotomy for the staging of Hodgkin's disease.

7. Decalcification for histochemical demonstration of hydrolytic and oxidative enzymes.

8. Bone marrow biopsy with unaltered architecture: a new biopsy device.

9. [Preparative prerequisites in clinical histology of human bone marrow. 1. Methodical studies on embedding in acrylates of lipid-rich tissue material].

10. The demonstration of anions generated by the action of sodium hypochlorite (NaOCl) on tissue sections, including observations on the unmasking of carbonyl groups.

1. 2-Hydroxyethyl methacrylate for sectioning bone marrow.

2. Cell suspensions from collagenase digestion of bone marrow trephine biopsy specimens.

Review 3. Bone biopsy in haematological disorders.