Literature DB >> 60264

Cellular localization of intestinal calcium-binding protein in pig duodenum.

B M Arnold, K Kovacs, T M Murray.   

Abstract

The gamma-globulin fraction of a rabbit antiserum against porcine intestinal calcium-binding protein (CaBP) was used in an immunoperoxidase method to study CaBP localization in porcine duodenal tissue. Specific immunostaining, indicative of the presence of CaBP, was noted within the cytoplasm of the duodenal epithelial cells. No CaBP was detected in goblet cells or in the subepithelial layers. When the specific antibody was replaced by either nonimmune rabbit gamma-globulin or when the specific antibody was preadsorbed with excess CaBP, no positive immunostaining was seen. Our studies lend support to the hypothesis that CaBP may function in intracellular calcium transport.

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Year:  1976        PMID： 60264     DOI： 10.1159/000197801

Source DB:  PubMed          Journal:  Digestion        ISSN： 0012-2823            Impact factor:   3.216


  7 in total

1.  The relationship between vitamin D-stimulated calcium transport and intestinal calcium-binding protein in the chicken.

Authors:  R Spencer; M Charman; P W Wilson; E M Lawson
Journal:  Biochem J       Date:  1978-01-15       Impact factor: 3.857

2.  Effects of 1 alpha-hydroxy vitamin D3 on the rachitic chick intestine: a comparison to the effects of 1,25-dihydroxyvitamin D3.

Authors:  D D Bikle; R N Empson; R L Morrissey; D T Zolock; T J Bucci; R H Herman; M M Pechet
Journal:  Calcif Tissue Int       Date:  1980       Impact factor: 4.333

3.  Immunocytochemical localization of a calcium-binding protein in the rat duodenum.

Authors:  P Marche; C Le Guern; P Cassier
Journal:  Cell Tissue Res       Date:  1979-03-09       Impact factor: 5.249

4.  Identification of intestinal cells responsive to calcitriol (1,25-dihydroxycholecalciferol).

Authors:  M W Smith; M E Bruns; E D Lawson
Journal:  Biochem J       Date:  1985-01-01       Impact factor: 3.857

5.  Vitamin-D-dependent calcium-binding protein (CaBP-9K) in rat growth cartilage.

Authors:  N Balmain; E Tisserand-Jochem; M Thomasset; P Cuisinier-Gleizes; H Mathieu
Journal:  Histochemistry       Date:  1986

6.  Immunohistochemical localization of vitamin D-dependent calcium-binding protein in duodenum, kidney, uterus and cerebellum of chickens.

Authors:  S S Jande; S Tolnai; D E Lawson
Journal:  Histochemistry       Date:  1981

7.  Immunocytochemical localization of the vitamin D-dependent calcium binding protein in chick duodenum.

Authors:  B Thorens; J Roth; A W Norman; A Perrelet; L Orci
Journal:  J Cell Biol       Date:  1982-07       Impact factor: 10.539
  7 in total

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