Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 The rapid, simple and improved preparation of high specific activity alpha-[32P]dATP and alpha-[32P]ATP.

Literature DB >> 600797

The rapid, simple and improved preparation of high specific activity alpha-[32P]dATP and alpha-[32P]ATP.

Abstract

An improved method is described for the rapid and simple preparation of alpha-[32P]dATP and alpha-[32P]ATP from 32Pi in good yields and with specific activities from 20 - 150 Ci/mmol. The two-step procedure involves the chemical synthesis of the mononucleotide followed by its enzymic conversion to the triphosphate with myokinase (EC 2.7.4.3) and pyruvate kinase (EC 2.7.1.40) in the presence of trace amounts of dATP or ATP to prime the reaction. The two steps are carried out in the same reaction flask and the only purification step required is a step-wise elution from a column of DEAE-cellulose.

Entities: Chemical Disease Gene

Mesh：

Substances：

Year: 1977 PMID： 600797 PMCID： PMC343245 DOI： 10.1093/nar/4.12.4347

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

5 in total

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

2. Improved synthesis of 32P-labelled 3',5'-cyclic GMP and other 3',5'-cyclic ribo- and deoxyribonucleotides of high specific activity.

Authors: R H Symons
Journal: Biochim Biophys Acta Date: 1973-09-14

3. The 3'-terminal nucleotide sequences of T7 DNA.

Authors: P T Englund
Journal: J Mol Biol Date: 1972-05-14 Impact factor: 5.469

4. Practical methods for the routine chemical synthesis of 32P-labelled nucleoside di- and triphosphates.

Authors: R H Symons
Journal: Biochim Biophys Acta Date: 1970

5. Synthesis of (alpha-32P) ribo- and deoxyribonucleoside 5'-triphosphates.

Authors: R H Symons
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

5 in total

7 in total

The rapid, simple and improved preparation of high specific activity alpha-[32P]dATP and alpha-[32P]ATP.

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

2. Improved synthesis of 32P-labelled 3',5'-cyclic GMP and other 3',5'-cyclic ribo- and deoxyribonucleotides of high specific activity.

3. The 3'-terminal nucleotide sequences of T7 DNA.

4. Practical methods for the routine chemical synthesis of 32P-labelled nucleoside di- and triphosphates.

5. Synthesis of (alpha-32P) ribo- and deoxyribonucleoside 5'-triphosphates.

1. Extensive sequence homology at the 3'-termini of the four RNAs of cucumber mosaic virus.

2. A method for the enzymatic synthesis and purification of [alpha-32P] nucleoside triphosphates.

3. Cleavage of DNA.RNA hybrids by type II restriction enzymes.

4. Differences in response to activation of adenylyl cyclase by various stimulants in human myocardium.

5. Identification and characterization of a new transcriptional termination factor from Escherichia coli.

6. Avocado sunblotch viroid: primary sequence and proposed secondary structure.

7. Organisation of feather keratin genes in the chick genome.