Literature DB >> 600796

A critical comparison of commonly used procedures for the assay of terminal deoxynucleotidyl transferase in crude tissue extracts.

M S Coleman.   

Abstract

Terminal deoxynucleotidyl transferase (TdT) is a non-template directed DNA polymerase normally found in vertebrate thymus and bone marrow. Quantitative assay of TdT activity is being widely used as a tool in the differential diagnosis of acute leukemias in man. Clinical specimens of blood and bone marrow often contain 10(7) or fewer cells and require a specific and rapid assay for transferase which can be carried out in crude cell extracts. Commonly used assay methods do not meet these requirements, but can be easily modified to do so.

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Year:  1977        PMID： 600796      PMCID： PMC343242          DOI： 10.1093/nar/4.12.4305

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  THE ENZYMATIC CONDENSATION OF OLIGODEOXYRIBONUCLEOTIDES WITH POLYDEOXYRIBONUCLEOTIDES.

Authors:  C G MEAD
Journal:  Proc Natl Acad Sci U S A       Date:  1964-12       Impact factor: 11.205

2.  Terminal deoxynucleotidyl transferase: characterization of extraction and assay conditions from human and calf tissue.

Authors:  M S Coleman
Journal:  Arch Biochem Biophys       Date:  1977-08       Impact factor: 4.013

3.  Is terminal deoxynucleotidyl transferase a somatic mutagen in lymphocytes?

Authors:  D Baltimore
Journal:  Nature       Date:  1974-03-29       Impact factor: 49.962

4.  DNA-dependent DNA polymerases I and II from normal human-blood lymphocytes.

Authors:  R G Smith; R C Gallo
Journal:  Proc Natl Acad Sci U S A       Date:  1972-10       Impact factor: 11.205

5.  DNA polymerases in normal and leukemic human hematopoietic cells.

Authors:  M S Coleman; J J Hutton; F J Bollum
Journal:  Blood       Date:  1974-07       Impact factor: 22.113

6.  Low molecular weight deoxyribonucleic acid polymerase from calf thymus chromatin. I. Preparation of homogeneous enzyme.

Authors:  L M Chang
Journal:  J Biol Chem       Date:  1973-06-10       Impact factor: 5.157

7.  Low molecular weight deoxyribonucleic acid polymerase from rabbit bone marrow.

Authors:  L M Chang; F J Bollum
Journal:  Biochemistry       Date:  1972-03-28       Impact factor: 3.162

8.  Terminal deoxynucleotidyl transferase in a case of childhood acute lymphoblastic leukemia.

Authors:  R McCaffrey; D F Smoler; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1973-02       Impact factor: 11.205

9.  Terminal deoxyribonucleotidyl transferase in human leukemia.

Authors:  M S Coleman; J J Hutton; P De Simone; F J Bollum
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205

10.  Terminal deoxynucleotidyl transferase measurements in the differential diagnosis of adult leukaemias.

Authors:  J J Hutton; M S Coleman
Journal:  Br J Haematol       Date:  1976-11       Impact factor: 6.998
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  7 in total

1.  Properties of a terminal deoxyribonucleotidyltransferase isolated from wheat germ.

Authors:  T Brodniewicz-Proba; J Buchowicz
Journal:  Biochem J       Date:  1980-10-01       Impact factor: 3.857

2.  Terminal deoxynucleotidyl transferase expression in a Thy-l alloantigen variant lymphoma cell line.

Authors:  T F Bumol; E F Retzel; S D Douglas; R S Basch; J N Buxbaum; A J Faras
Journal:  Immunology       Date:  1980-12       Impact factor: 7.397

3.  Biochemical and immunological properties of human terminal deoxynucleotidyl transferase purified from blasts of acute lymphoblastic and chronic myelogenous leukemia.

Authors:  M R Deibel; M S Coleman; K Acree; J J Hutton
Journal:  J Clin Invest       Date:  1981-03       Impact factor: 14.808

4.  Detection of terminal transferase in paraffin sections with the immunoperoxidase technique.

Authors:  C A Halverson; B Falini; C R Taylor; J W Parker
Journal:  Am J Pathol       Date:  1981-12       Impact factor: 4.307

5.  Terminal uridylyl transferase of Vigna unguiculata: purification and characterization of an enzyme catalyzing the addition of a single UMP residue to the 3'-end of an RNA primer.

Authors:  P Zabel; L Dorssers; K Wernars; A Van Kammen
Journal:  Nucleic Acids Res       Date:  1981-06-11       Impact factor: 16.971

6.  The two isoforms of mouse terminal deoxynucleotidyl transferase differ in both the ability to add N regions and subcellular localization.

Authors:  L A Bentolila; M Fanton d'Andon; Q T Nguyen; O Martinez; F Rougeon; N Doyen
Journal:  EMBO J       Date:  1995-09-01       Impact factor: 11.598

7.  Terminal deoxynucleotidyl transferase in human lymphomas: possible existence of forms with high and low molecular weights.

Authors:  P Vezzoni; F Campagnari; G Di Fronzo; L Clerici
Journal:  Br J Cancer       Date:  1981-03       Impact factor: 7.640
  7 in total

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