Literature DB >> 598849

Rat hepatocyte primary cell cultures. III. Improved dissociation and attachment techniques and the enhancement of survival by culture medium.

G M Williams, E Bermudez, D Scaramuzzino.   

Abstract

The conditions for obtaining representative, adult rat hepatocyte primary cell cultures were improved such that viable yields of 50% of the liver were produced which gave rise to cultures representing 30% of the liver. The survival of these cultures in various media was compared revealing that in complex media, particularly containing galactose, survival was improved.

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Year:  1977        PMID： 598849     DOI： 10.1007/bf02615128

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  33 in total

1.  Primary culture of parenchymal liver cells on collagen membranes. Morphological and biochemical observations.

Authors:  G Michalopoulos; H C Pitot
Journal:  Exp Cell Res       Date:  1975-08       Impact factor: 3.905

2.  THE GROWTH AND MAINTENANCE OF TISSUE-CELL CULTURES IN FREE GAS EXCHANGE WITH THE ATMOSPHERE.

Authors:  A LEIBOVITZ
Journal:  Am J Hyg       Date:  1963-09

3.  The utilization of carbohydrates by human cell cultures.

Authors:  H EAGLE; S BARBAN; M LEVY; H O SCHULZE
Journal:  J Biol Chem       Date:  1958-09       Impact factor: 5.157

4.  Propagation of conjunctival and HeLa cells in various carbohydrate media.

Authors:  R S CHANG; R P GEYER
Journal:  Proc Soc Exp Biol Med       Date:  1957-11

5.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

6.  Primary culture of normal adult rat liver cells which maintain stable urea cycle enzymes.

Authors:  R C Lin; P J Snodgrass
Journal:  Biochem Biophys Res Commun       Date:  1975-05-19       Impact factor: 3.575

Review 7.  "Feeding the baby"--designing the culture milieu to enhance cell stability.

Authors:  C Waymouth
Journal:  J Natl Cancer Inst       Date:  1974-11       Impact factor: 13.506

8.  Respiratory activity of intact, isolated parenchymal cells from rat liver.

Authors:  R B Howard; L A Pesch
Journal:  J Biol Chem       Date:  1968-06-10       Impact factor: 5.157

9.  Primary monolayer cultures of adult rat liver parenchymal cells suitable for study of the regulation of enzyme synthesis.

Authors:  R J Bonney; J E Becker; P R Walker; V R Potter
Journal:  In Vitro       Date:  1974 May-Jun

10.  Morphology and function of cells of human embryonic liver in monolayer culture.

Authors:  D M Bissell; J G Tilles
Journal:  J Cell Biol       Date:  1971-07       Impact factor: 10.539
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  58 in total

1.  Primary cultures of hepatocytes on human fibroblasts.

Authors:  G Michalopoulos; F Russell; C Biles
Journal:  In Vitro       Date:  1979-10

Review 2.  Sandwich-cultured hepatocytes: an in vitro model to evaluate hepatobiliary transporter-based drug interactions and hepatotoxicity.

Authors:  Brandon Swift; Nathan D Pfeifer; Kim L R Brouwer
Journal:  Drug Metab Rev       Date:  2010-08       Impact factor: 4.518

3.  Crude liver membrane fractions as substrate preserve liver-specific functions in long-term, serum-free rat hepatocyte cultures.

Authors:  B Saad; H Schawalder; P Maier
Journal:  In Vitro Cell Dev Biol       Date:  1993-01

4.  Isolation of rat hepatocytes with EDTA and their metabolic functions in primary culture.

Authors:  S R Wang; G Renaud; J Infante; D Catala; R Infante
Journal:  In Vitro Cell Dev Biol       Date:  1985-09

5.  Sex differences in the biotransformation of 2-acetylaminofluorene in cultured rat hepatocytes.

Authors:  C A McQueen; M J Miller; G M Williams
Journal:  Cell Biol Toxicol       Date:  1986-06       Impact factor: 6.691

6.  In vitro induction of unscheduled DNA synthesis by genotoxic carcinogens in the hepatocytes of the oyster toadfish (Opsanus tau).

Authors:  J J Kelly; M B Maddock
Journal:  Arch Environ Contam Toxicol       Date:  1985-09       Impact factor: 2.804

7.  Properties of peroxisomes and their induction by clofibrate in normal adult rat hepatocytes in primary culture.

Authors:  K Furukawa; Y Mochizuki; N Sawada
Journal:  In Vitro       Date:  1984-07

8.  Relationship between the genetically determined acetylator phenotype and DNA damage induced by hydralazine and 2-aminofluorene in cultured rabbit hepatocytes.

Authors:  C A McQueen; C J Maslansky; I B Glowinski; S B Crescenzi; W W Weber; G M Williams
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

9.  Induction of peroxisome proliferation in hepatocytes transplanted into the anterior chamber of the eye. A model system for the evaluation of xenobiotic-induced effects.

Authors:  M S Rao; S Thorgeirsson; M K Reddy; N D Lalwani; R E Evarts; M I Usman; B Singh; J K Reddy
Journal:  Am J Pathol       Date:  1986-09       Impact factor: 4.307

10.  Comparative metabolism and genotoxicity of the structurally similar nitrophenylenediamine dyes, HC Blue 1 and HC Blue 2, in mouse hepatocytes.

Authors:  F W Kari; S M Driscoll; A Abu-Shakra; S C Strom; W L Jenkins; J S Volosin; K M Rudo; R Langenbach
Journal:  Cell Biol Toxicol       Date:  1990-04       Impact factor: 6.691
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