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Literature DB >> 59719

Localization of glycoidases with naphthyl substrates.

Abstract

Unsubstituted naphthyl substrates were found to be superior to substituted naphthyl, indolyl and hydroxyquinoline substrates for the histochemical demonstration of alpha-mannosidase, alpha-galactosidase, hetero-beta-glycosidase, glucoamylase and sucraseisomaltase, equivalent for beta-N-acetylglucosaminidase and lactase-beta-glucosidase, and inferior for beta-glucuronidase and acid beta-galatosidase. Aldehyde fixation is necessary for the localization of lysosomal glycosidases with naphthyl substrates. 1-naphthyl substrates are suitable for the detection of acid glycosidases in lysosomes and hetero-beta-glysocidase in the cytoplasm of animal cells, and 2-naphthyl substrates can be employed for the demonstration of microvillous glycosidases and for the evaluation of the total activity of soluble glycosidases with semipermeable membranes. When naphthyl substrates are used coupling should be carried out simultaneously and hexazotized pararosaniline is the coupling reagent of choice.

Entities: Chemical Gene

Mesh：

Substances：

Year: 1976 PMID： 59719 DOI： 10.1007/bf01003816

Source DB: PubMed Journal: Histochem J ISSN： 0018-2214

46 in total

10. Human small-intestinal beta-galactosidases. Separation and characterization of one lactase and one hetero beta-galactosidase.

Authors: N G Asp; A Dahlqvist; O Koldovský
Journal: Biochem J Date: 1969-09 Impact factor: 3.857

11 in total

4. A quantitative histochemical technique for the characterisation of alpha-glucosidases in the brush-border membrane of rat jejunum.

Authors: S Gutschmidt; W Kaul; E O Riecken
Journal: Histochemistry Date: 1979-09

5. A family of at least seven beta-galactosidase genes is expressed during tomato fruit development.

Authors: D L Smith; K C Gross
Journal: Plant Physiol Date: 2000-07 Impact factor: 8.340

6. Enzymatic activity and substrate specificity of recombinant tomato beta-galactosidases 4 and 5.

Authors: Megumi Ishimaru; David L Smith; Andrew J Mort; Kenneth C Gross
Journal: Planta Date: 2008-11-06 Impact factor: 4.116

7. [Azoindoxyl methods for the investigation of hydrolases. IV. Suitability of various diazonium salts (author's transl)].

Authors: R Gossrau
Journal: Histochemistry Date: 1978-09-28

8. [Histochemical and biochemical investigation of alpha-glucosidases by means of 2-naphthyl-alpha-D-glucoside (author's transl)].

Authors: R Gossrau
Journal: Histochemistry Date: 1976-10-29

9. Quantitative cytochemistry of beta-galactosidase in normal and enzyme deficient (gal) pollen of Brassica campestris: application of the indigogenic method.

Authors: M B Singh; R B Knox
Journal: Histochem J Date: 1984-12

10. [Peptidases. I. Histochemical investigations with 2-naphthylamides and hexazonium-p-rosanilin (author's transl)].

Authors: R Gossrau
Journal: Histochemistry Date: 1977-12-28

Localization of glycoidases with naphthyl substrates.

1. Significance of coupling rate in histochemical azo dye methods for enzymes.

2. Histochemical demonstration of beta-D-galactosidase in the rat.

3. Histochemical demonstration of beta-d-glucuronidase with a synthetic substrate.

4. The fine localization of N-acetyl- -glucosaminidase in rat tissues using an indoxyl substrate.

5. Synthetic substrates in the histochemical demonstration of intestinal disaccharidases.

6. [Histochemistry and microchemistry in the nephron].

7. The preparation of lactase and glucoamylase of rat small intestine.

8. Assay of 2-naphthyl beta-galactosidase activity.

9. [The application of histological and histochemical techniques to freeze-dried cryostat sections].