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Literature DB >> 596935

Biochemical characterization of connective tissue macromolecules derived from cutaneous fibrosarcoma.

Abstract

Tumor tissue of cutaneous fibrosarcoma was solubilized with salt, acetic acid and salt-extracted after pepsin treatment. Type III collagen was observed in neutral soluble collagen, 1.5 M and 2.4 M NaCl precipitated fractions after pepsin treatment. Type III collagen fraction precipitated with 1.5 M NaCl eluted in the alpha2 region when chromatographed on CM-cellulose without reduction, while the type III collagen partially purified with 1.5 M NaCl eluted between the position of alpha1(I) and beta12 after reduction and alkylation. Analysis of amino acid showed the presence of cysteine and the high content of hydroxyproline following CM-chromatography of 1.5 M NaCl precipitated type III collagen fraction. Acidic glycosaminoglycans were composed of hyaluronic acid, chondroitin sulfate, dermatan sulfate and heparan sulfate, and the first two were major components of fibrosarcoma.

Entities: Chemical Disease

Mesh：

Substances：
Collagen

Year: 1977 PMID： 596935 DOI： 10.1007/bf00561114

Source DB: PubMed Journal: Arch Dermatol Res Impact factor: 3.017

19 in total

1. Isolation, chemical and electron microscopical characterization of neutral-salt-soluble type III collagen and procollagen from fetal bovine skin.

Authors: R Timpl; R W Glanville; H Nowack; H Wiedemann; P P Fietzek; K Kühn
Journal: Hoppe Seylers Z Physiol Chem Date: 1975-11

Biochemical characterization of connective tissue macromolecules derived from cutaneous fibrosarcoma.

1. Isolation, chemical and electron microscopical characterization of neutral-salt-soluble type III collagen and procollagen from fetal bovine skin.

2. Type III collagen: A major constituent of rheumatoid and normal human synovial membrane.

3. [The polymorphism of collagen. New viewpoints on the structure and function of connective tissue].

4. Isolation and characterization of the cyanogen bromide peptides from the alpha 1(3) chain of human collagen.

5. (Alpha1(3))3 human skin collagen. Release by pepsin digestion and preponderance in fetal life.

6. A rapid and micro method for separation of acidic glycosaminoglycans by two-dimensional electrophoresis.

7. Skin procollagen peptidase in normal and pathologic conditions.

8. Collagen polymorphism: characterization of molecules with the chain composition (alpha 1 (3)03 in human tissues.

9. Isolation and characterization of type III collagen from chick stain.

10. Increase in type I and type III collagens in human alcoholic liver cirrhosis.

1. Characterization of the synthesized collagen by mouse L-929 (L-5) cells.

Review 2. Collagen metabolism: a comparison of diseases of collagen and diseases affecting collagen.

3. Nature of collagen in dermatofibrosarcoma protuberans.