Pyruvate-carbon dioxide exchange reaction of Desulfovibrio desulfuricans.

Suh, Byungse (University of Kansas, Lawrence), and J. M. Akagi. Pyruvate-carbon dioxide exchange reaction of Desulfovibrio desulfuricans. J. Bacteriol. 91:2281-2285. 1966.-The pyruvate-CO(2) exchange reaction, catalyzed by Desulfovibrio desulfuricans, required the presence of phosphate and coenzyme A. However, the requirement for phosphate disappeared when the concentration of coenzyme A was increased to a level of 3.8 x 10(-3)m. Passing crude extracts through a diethylaminoethyl-cellulose column and an Amberlite CG-50 ion-exchange column, to remove ferredoxin and cytochrome c(3), resulted in a marked decrease in exchange activity; full activity was restored by the addition of ferredoxin or cytochrome c(3). Fe(++) or Co(++) stimulated the exchange of CO(2) into pyruvate.