Literature DB >> 593893

Purification and properties of tyrosyl tRNA synthetase of rat liver.

Y S Prasada Rao, P R Srinivasan.   

Abstract

Purification of rat liver tyrosine tRNA synthetase yields two protein fractions A and B and both fractions are required for charging of tyrosine to tRNAtyr. Fraction B catalyzes the activation of tyrosine. Fractions A and B have been purified to near homogeneity and they are composed of single polypeptide chains of 62,000 daltons each. Gel filtration studies suggest a molecular weight of 120,000 for the synthetase.

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Year:  1977        PMID： 593893      PMCID： PMC343208          DOI： 10.1093/nar/4.11.3887

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

1.  The acetate activating enzyme system of heart muscle.

Authors:  H BEINERT; D E GREEN; P HELE; H HIFT; R W VON KORFF; C V RAMAKRISHNAN
Journal:  J Biol Chem       Date:  1953-07       Impact factor: 5.157

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Tyroslyl-tRNA synthetase from baker's yeast. Rapid isolation by affinity elution, molecular weight of the enzyme, and determination of essential sulfhydryl groups.

Authors:  H G Faulhammer; F Cramer
Journal:  Eur J Biochem       Date:  1977-05-16

4.  Glutamyl transfer ribonucleic acid synthetase of Escherichia coli. 3. Influence of the 46K protein on the affinity of the 56K glutamyl transfer ribonucleic acid synthetase for its substrates.

Authors:  J Lapointe; D Söll
Journal:  J Biol Chem       Date:  1972-08-25       Impact factor: 5.157

5.  Purification of tyrosine: tRNA ligase from Saccharomyces cerevisiae alphaS288C.

Authors:  Z Kućan; R W Chambers
Journal:  J Biochem       Date:  1973-04       Impact factor: 3.387

6.  Tyrosyl-tRNA synthetase of Escherichia coli B. Binding of various ligands.

Authors:  S Chousterman; F Chapeville
Journal:  Eur J Biochem       Date:  1973-05

7.  Tyrosyl-tRNA synthetase from baker's yeast. Isolation and some properties.

Authors:  H Beikirch; F von der Haar; F Cramer
Journal:  Eur J Biochem       Date:  1972-03-27

8.  Glutamyl transfer ribonucleic acid synthetase of Escherichia coli. I. Purification and properties.

Authors:  J Lapointe; D Söll
Journal:  J Biol Chem       Date:  1972-08-25       Impact factor: 5.157

9.  Tyrosyl transfer ribonucleic acid synthetase from Bacillus stearothermophilus. Preparation and properties of the crystallizable enzyme.

Authors:  G L Koch
Journal:  Biochemistry       Date:  1974-05-21       Impact factor: 3.162

10.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.

Authors:  A L Shapiro; E Viñuela; J V Maizel
Journal:  Biochem Biophys Res Commun       Date:  1967-09-07       Impact factor: 3.575
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