| Literature DB >> 591723 |
T B Higerd, G Virella, R Cardenas, J Koistinen, J W Fett.
Abstract
A simple method for obtaining an active preparation of IgA-specific protease from a bacterial source is presented. In this method Streptococcus sanguis was inoculated onto the surface of a dialysis membrane on nutrient agar. Following growth, the membrane was removed from the agar surface and washed in a small volume of buffer. A solution with protease activity against IgA1 monoclonal proteins was obtained by clarification of the wash and appeared to be similar to enzyme preparations obtained by other methods.Entities:
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Year: 1977 PMID： 591723 DOI： 10.1016/0022-1759(77)90178-8
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303