Literature DB >> 591514

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase.

T Morita, H Kato, S Iwanaga, K Takada, T Kimura.   

Abstract

Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides contained arginine-MCA as the carboxyl-terminus. Release of 7-amino-4-methylcoumarin was determined fluorometrically. Of these peptides, the following were found to be specific substrates for individual enzymes: Boc-Val-Pro-Arg-MCA for alpha-thrombin, Boc-Ile-Glu-Gly-Arg-MCA, and Boc-Ser-Gly-Arg-MCA for factor Xa, Z-Phe-Arg-MCA for plasma kallikrein, Pro-Phe-Arg-MCA for pancreatic and urinary kallikreins, and glutaryl-Gly-Arg-MCA for urokinase. Moreover, these peptide-MCA substrates were resistant to plasmin.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 591514     DOI： 10.1093/oxfordjournals.jbchem.a131840

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  53 in total

1.  Collagenolytic serine-carboxyl proteinase from Alicyclobacillus sendaiensis strain NTAP-1: purification, characterization, gene cloning, and heterologous expression.

Authors:  Naoki Tsuruoka; Toru Nakayama; Masako Ashida; Hisashi Hemmi; Masahiro Nakao; Hiroyuki Minakata; Hiroshi Oyama; Kohei Oda; Tokuzo Nishino
Journal:  Appl Environ Microbiol       Date:  2003-01       Impact factor: 4.792

2.  Printing chemical libraries on microarrays for fluid phase nanoliter reactions.

Authors:  Dhaval N Gosalia; Scott L Diamond
Journal:  Proc Natl Acad Sci U S A       Date:  2003-07-08       Impact factor: 11.205

3.  Optimization of cell culture conditions for production of biologically active proteins.

Authors:  S Hosoi; H Miyaji; M Satoh; T Kurimoto; A Mihara; N Fujiyoshi; S Itoh; S Sato
Journal:  Cytotechnology       Date:  1991-01       Impact factor: 2.058

4.  Protein association and dissociation regulated by extension peptide: a mode for iron control by phytoferritin in seeds.

Authors:  Haixia Yang; Xiaoping Fu; Meiliang Li; Xiaojing Leng; Bin Chen; Guanghua Zhao
Journal:  Plant Physiol       Date:  2010-09-14       Impact factor: 8.340

5.  Increased secretion of glandular-kallikrein in the bronchial washings induced by intravenous injection of leukotriene C4 in guinea-pigs.

Authors:  H Y Jin; M Katori; M Majima; N Sunahara
Journal:  Br J Pharmacol       Date:  1992-03       Impact factor: 8.739

6.  MX1013, a dipeptide caspase inhibitor with potent in vivo antiapoptotic activity.

Authors:  Wu Yang; John Guastella; Jin-Cheng Huang; Yan Wang; Li Zhang; Dong Xue; Minhtam Tran; Richard Woodward; Shailaja Kasibhatla; Ben Tseng; John Drewe; Sui Xiong Cai
Journal:  Br J Pharmacol       Date:  2003-08-26       Impact factor: 8.739

7.  Localization of kallikrein-like activity along a single nephron in rabbits.

Authors:  K Tomita; H Endou; F Sakai
Journal:  Pflugers Arch       Date:  1981-01       Impact factor: 3.657

8.  Human mannose-binding lectin inhibitor prevents myocardial injury and arterial thrombogenesis in a novel animal model.

Authors:  Vasile I Pavlov; Ying S Tan; Erin E McClure; Laura R La Bonte; Chenhui Zou; William B Gorsuch; Gregory L Stahl
Journal:  Am J Pathol       Date:  2014-12-04       Impact factor: 4.307

9.  Activation of mannan-binding lectin-associated serine proteases leads to generation of a fibrin clot.

Authors:  Krishana C Gulla; Kshitij Gupta; Anders Krarup; Peter Gal; Wilhelm J Schwaeble; Robert B Sim; C David O'Connor; Krishnan Hajela
Journal:  Immunology       Date:  2009-12-02       Impact factor: 7.397

10.  The chemical mediation of delayed hypersensitivity skin reactions. IV. Activation of chemotactic factor precursor by a trypsin-like protease in guinea pig plasma.

Authors:  T Kawaguchi; K Ueda; T Yamamoto; T Kambara
Journal:  Am J Pathol       Date:  1984-05       Impact factor: 4.307
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.