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Literature DB >> 5911529

Properties of a purified rat-liver nuclease.

Abstract

1. The pH optimum, ionic requirement and heat-stability of a purified liver nuclease have been examined with RNA and denatured DNA as substrates. 2. The enzyme attacked DNA and RNA in an endonucleolytic manner, forming oligonucleotides terminated by 5'-phosphate groups. No clear specificity was found with respect to the bases at the site of cleavage. 3. Comparison of the results obtained with RNA and denatured DNA as substrates suggests that the ribonuclease and deoxyribonuclease activities are associated with the same protein.

Entities: Chemical Species

Mesh：

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Year: 1966 PMID： 5911529 PMCID： PMC1264924 DOI： 10.1042/bj0980818

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

12 in total

1. CHARACTERIZATION OF A PHOSPHODIESTERASE FROM LAMB BRAIN.

Authors: J W HEALY; D STOLLAR; M I SIMON; L LEVINE
Journal: Arch Biochem Biophys Date: 1963-12 Impact factor: 4.013

2. PURIFICATION OF ALKALINE RIBONUCLEASE II FROM MITOCHONDRIAL AND SOLUBLE FRACTIONS OF LIVER.

Authors: J R BEARD; W E RAZZELL
Journal: J Biol Chem Date: 1964-12 Impact factor: 5.157

3. Mechanism of action of micrococcal nuclease on deoxyribonucleic acid.

Authors: E SULKOWSKI; M LASKOWSKI
Journal: J Biol Chem Date: 1962-08 Impact factor: 5.157

4. Purification of phosphodiesterase from Bothrops atrox venom, with special consideration of the elimination of monophosphatases.

Authors: W BJORK
Journal: J Biol Chem Date: 1963-07 Impact factor: 5.157

2. The preference of the mitochondrial endonuclease for a conserved sequence block in mitochondrial DNA is highly conserved during mammalian evolution.

Authors: R L Low; J M Buzan; C L Couper
Journal: Nucleic Acids Res Date: 1988-07-25 Impact factor: 16.971

2 in total

Properties of a purified rat-liver nuclease.

1. CHARACTERIZATION OF A PHOSPHODIESTERASE FROM LAMB BRAIN.

2. PURIFICATION OF ALKALINE RIBONUCLEASE II FROM MITOCHONDRIAL AND SOLUBLE FRACTIONS OF LIVER.

3. Mechanism of action of micrococcal nuclease on deoxyribonucleic acid.

4. Purification of phosphodiesterase from Bothrops atrox venom, with special consideration of the elimination of monophosphatases.

5. Action of venom phosphodiesterase on deoxyribonucleic acid.

6. THE DEOXYRIBONUCLEASES OF STREPTOCOCCUS PYOGENS.

7. Ribonuclease. VI. Partial purification and characterization of the ribonucleases of rat liver mitochondria.

8. On the mechanism of metal activation of deoxyribobuclease I.

9. The purification from rat liver of a nuclease hydrolysing ribonucleic acid and deoxyribonucleic acid.

10. Action of venom phosphodiesterase on deoxyribooligonucleotides carrying a monoesterified phosphate on carbon 3'.

1. Purification and properties of a single strand-specific endonuclease from mouse cell mitochondria.

2. The preference of the mitochondrial endonuclease for a conserved sequence block in mitochondrial DNA is highly conserved during mammalian evolution.