| Literature DB >> 5911523 |
Abstract
1. Various methods for the extraction of choline acetyltransferase (acetyl-CoA-choline O-acetyltransferase, EC 2.3.1.6) from immature human placenta (18-28 weeks of gestation) are described. 2. The crude enzyme was found to be stable at -18 degrees and +4 degrees under a variety of conditions. 3. Purification methods, including ammonium sulphate fractionation, gel filtration on various grades of Sephadex and DEAE-Sephadex fractionation, have yielded a preparation of high specific activity.Entities:
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Year: 1966 PMID: 5911523 PMCID: PMC1264916 DOI: 10.1042/bj0980754
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857