Literature DB >> 5909582

Autoradiography with tritiated methotrexate and the cellular distribution of folate reductase.

Z Darzynkiewicz, A W Rogers, E A Barnard, D H Wang, W C Werkheiser.   

Abstract

Bound methotrexate has been revealed by an autoradiographic procedure, presumed to introduce a method for cytochemical study of folate reductase. Preferential localization is seen in kidney proximal tubules, intestinal epithelium, and nuclei of parenchymal liver cells in mice. The extremely firm binding and prolonged retention of this drug should render it suitable as an inert label for the autoradiographic study of cell migrations and lifetimes.

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Year:  1966        PMID： 5909582     DOI： 10.1126/science.151.3717.1528

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  3 in total

Review 1.  The labelled inhibitor method in cytoenzymology.

Authors:  K Ostrowski
Journal:  Histochem J       Date:  1972-09

Review 2.  Use of fluorescently labeled caspase inhibitors as affinity labels to detect activated caspases.

Authors:  Jerzy Grabarek; Paul Amstad; Zbigniew Darzynkiewicz
Journal:  Hum Cell       Date:  2002-03       Impact factor: 4.174

3.  Quantitative studies on enzymes in structures in striated muscles by labeled inhibitor methods. I. The number of acetylcholinesterase molecules and of other DFP-reactive sites at motor endplates, measured by radioautography.

Authors:  A W Rogers; Z Darzynkiewicz; M M Salpeter; K Ostrowski; E A Barnard
Journal:  J Cell Biol       Date:  1969-06       Impact factor: 10.539
  3 in total

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