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Literature DB >> 590742

A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus.

A Kiss, B Sain, E Csordás-Tòth, P Venetianer.

Abstract

A new restriction endonuclease has been isolated from Bacillus sphaericus R. The purification procedure includes Bio-Gel filtration, (NH4)2SO4 fractionation and phosphocellulose chromatography. After the phosphocellulose step the enzyme preparation is free of non-specific nucleases. Bsp cleaves double-stranded DNA with the same specificity as Bacillus subtilis (Bsu) and Haemophilus aegyptius (HaeIII) restriction endonucleases, as concluded from digests and double-digests of phiX174 replicative form DNA with Bsu and Bsp. The 5'-terminal nucleotide of the cleavage products was shown to be C. Bacillus sphaericus R produces Bsp in extremely large quantities and the enzyme can be easily purified in high yield.

Entities: Chemical Species

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Year: 1977 PMID： 590742 DOI： 10.1016/0378-1119(77)90037-3

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

20 in total

1. A new Thermus sp. class-IIS enzyme sub-family: isolation of a 'twin' endonuclease TspDTI with a novel specificity 5'-ATGAA(N(11/9))-3', related to TspGWI, TaqII and Tth111II.

Authors: Piotr M Skowron; Jarosław Majewski; Agnieszka Zylicz-Stachula; Sylwia M Rutkowska; Izabela Jaworowska; Renata I Harasimowicz-Słowińska
Journal: Nucleic Acids Res Date: 2003-07-15 Impact factor: 16.971

2. Restriction enzymes and their isoschizomers.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1990-04-25 Impact factor: 16.971

3. Restriction enzymes and their isoschizomers.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1989 Impact factor: 16.971

4. Restriction and modification enzymes and their recognition sequences.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1985 Impact factor: 16.971

5. Restriction enzymes and their isoschizomers.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1988 Impact factor: 16.971

6. Reconstitution of mononucleosomes: characterization of distinct particles that differ in the position of the histone core.

Authors: W Linxweiler; W Hörz
Journal: Nucleic Acids Res Date: 1984-12-21 Impact factor: 16.971

A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus.

1. A new Thermus sp. class-IIS enzyme sub-family: isolation of a 'twin' endonuclease TspDTI with a novel specificity 5'-ATGAA(N(11/9))-3', related to TspGWI, TaqII and Tth111II.

2. Restriction enzymes and their isoschizomers.

3. Restriction enzymes and their isoschizomers.

4. Restriction and modification enzymes and their recognition sequences.

5. Restriction enzymes and their isoschizomers.

6. Reconstitution of mononucleosomes: characterization of distinct particles that differ in the position of the histone core.

7. Complete sequence of pSC101.

8. Accessibility of expressed and non-expressed genes to a restriction nuclease.

9. Restriction and modification enzymes and their recognition sequences.

10. BspRI restriction endonuclease: cloning, expression in Escherichia coli and sequential cleavage mechanism.