Literature DB >> 5891219

Activity of beta-galactosidase in homogenates and isolated microvilli fraction of jejunal mucosa from suckling rats.

O Koldovský, R Noack, G Schenk, V Jirsová, A Heringová, H Braná.   

Abstract

1. beta-Galactosidase activity was studied in homogenates and isolated microvilli fraction of jejunal mucosa from 14-day-old suckling rats. o-Nitrophenyl beta-d-galactoside served as the substrate. 2. The microvilli fraction contains about one-third of the total activity of the original homogenate. 3. The pH optimum of the beta-galactosidase was 3.5 in the total homogenate and supernatant fraction, whereas in the microvilli fraction the maximum activity was at pH5.5. 4. This work gives further support to the view that two beta-galactosidases exist in the jejunal mucosa.

Entities:  

Mesh：

Substances：

Year:  1965        PMID： 5891219      PMCID： PMC1207064          DOI： 10.1042/bj0960492

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  4 in total

1.  CHANGES IN THE INDUCIBILITY OF GALACTOKINASE AND BETA-GALACTOSIDASE DURING INHIBITION OF GROWTH IN ESCHERICHIA COLI.

Authors:  K PAIGEN
Journal:  Biochim Biophys Acta       Date:  1963-10-01

2.  Studies of small intestine during development. II. The intracellular location of intestinal beta-galactosidase.

Authors:  R G DOELL; N KRETCHMER
Journal:  Biochim Biophys Acta       Date:  1963-03-12

3.  Adaptation to lactose.

Authors:  L A HERZENBERG; L A HERZENBERG
Journal:  Nutr Rev       Date:  1959-03       Impact factor: 7.110

4.  POSTNATAL DEVELOPMENT OF BETA-GALACTOSIDASE ACTIVITY IN THE SMALL INTESTINE OF THE RAT. EFFECT OF ADRENALECTOMY AND DIET.

Authors:  O KOLDOVSKY; F CHYTIL
Journal:  Biochem J       Date:  1965-01       Impact factor: 3.857
  4 in total
  5 in total

1.  Studies on the brush border membrane of the mouse duodenum. I. Membrane isolation and analysis of protein components.

Authors:  T Billington; P R Nayudu
Journal:  J Membr Biol       Date:  1975-04-23       Impact factor: 1.843

2.  Rat small-intestinal beta-galactosidases. Separation by ion-exchange chromatography and gel filtration.

Authors:  N G Asp; A Dahlqvist
Journal:  Biochem J       Date:  1968-02       Impact factor: 3.857

3.  Intestinal disaccharidase activities in the chick.

Authors:  R C Siddons
Journal:  Biochem J       Date:  1969-03       Impact factor: 3.857

4.  Characteristics of beta-galactosidase in the mucosa of the small intestine of infant rats. Physicochemical properties.

Authors:  J Kraml; O Koldovský; A Heringová; V Jirsová; K Kácl; M Ledvina; H Pelichová
Journal:  Biochem J       Date:  1969-09       Impact factor: 3.857

5.  Rat small-intestinal beta-galactosidases. Influence of pH on the hydrolysis of different substrates.

Authors:  A Dahlqvist; N G Asp
Journal:  Biochem J       Date:  1967-04       Impact factor: 3.857
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.