Role of ribonucleic acid biosynthesis in multiplication of type 5 adenovirus.

Flanagan, John F. (University of Pennsylvania, Philadelphia), and Harold S. Ginsberg. Role of ribonucleic acid biosynthesis in multiplication of type 5 adenovirus. J. Bacteriol. 87:977-987. 1964.-The requirement for ribonucleic acid (RNA) biosynthesis in the multiplication of type 5 adenovirus was investigated by using radioactive phosphorus to label nucleic acids and two pyrimidine analogues, 6-azauridine and 5-fluorouracil or 5-fluorouridine, to inhibit synthesis of functional RNA. The data indicate that biosynthesis of RNA after infection is essential for production of virus-specific deoxyribonucleic acid, virus antigens, and infectious particles. The onset of essential RNA synthesis occurs 8 to 9 hr after virus infection and prior to the biosynthesis of other known virus-induced macromolecules.