Growth of Allescheria boydii in anitbiotic-containing media.

Cazin, John, Jr. (University of Iowa, Iowa City), and David W. Decker. Growth of Allescheria boydii in antibiotic-containing media. J. Bacteriol. 90:1308-1313. 1965.-Thirteen isolates of Allescheria boydii were surveyed for their ability to grow and sporulate on media containing cycloheximide and chloramphenicol. The fungus grew well in the presence of 4 to 8 mg/ml of cycloheximide, whereas ascocarps and coremia were always inhibited at a concentration of the drug lower than that required to inhibit growth and conidial production. In certain strains, ascocarp production was inhibited by concentrations of cycloheximide (0.4 mg/ml) normally used in selective media; however, two strains produced mature ascocarps at a concentration of 1 mg/ml of the antibiotic, and two strains produced immature ascocarps at a concentration of 4 mg/ml. Growth inhibition was not observed with concentrations of chloramphenicol as high as 1,000 mg per liter in any of eight strains tested.