Modulation of noradrenaline incorporation by nerve activities in the rat submaxillary gland.

1. Rats were anaesthetized with chloralose and prepared for electrical stimulation of the cervical sympathetic trunk. The effect of such stimulation was studied on the incorporation of intravenously infused [(3)H]noradrenaline, [(3)H]adrenaline or [(3)H]dopamine by the submaxillary gland.2. A train of 200 stimuli every min for 30 min at 20/sec increased the total incorporation of [(3)H]noradrenaline by 92% over the unstimulated side of the gland, at 50/sec by about 200% and at 3.3/sec by about 30%. The incorporation of [(3)H]adrenaline and of [(3)H]dopamine was also increased by sympathetic stimulation.3. The increases in unaltered [(3)H]noradrenaline and its metabolites were proportional to the increase in total radioactivity.4. Mean venous outflow from the gland was decreased by 15-30% by the sympathetic stimulation.5. After elimination of extraneuronal binding of noradrenaline by ligation of the common excretory ducts, nerve stimulation still increased incorporation.6. Inhibition of normetanephrine production by pyrogallol did not antagonize the nerve impulse-mediated increase of [(3)H]noradrenaline incorporation. alpha-Methyltyrosine was also without effect.7. Desmethylimipramine and bretylium completely abolished the nerve impulse-mediated increase of [(3)H]noradrenaline incorporation in concentrations which did not affect the control uptake or abolish adrenergic transmission.8. Reserpine, guanethidine and phenoxybenzamine antagonized both the control and nerve impulse-mediated incorporations of [(3)H]noradrenaline.9. The results indicate that nerve impulses modulate the incorporation of transmitter amine at the neuronal membrane. Possible mechanisms for this modulation are discussed.