Literature DB >> 5812939

Pyruvate carboxylase in lactating rat and rabbit mammary gland.

B Gul, R Dils.   

Abstract

1. Pyruvate carboxylase [pyruvate-carbon dioxide ligase (ADP), EC 6.4.1.1] was found in cell-free preparations of lactating rat and rabbit mammary glands, and optimum assay conditions for this enzyme were determined. 2. Subcellular-fractionation studies with marker enzymes showed pyruvate carboxylase to be distributed between the mitochondrial and soluble fractions of lactating rat mammary gland. Evidence is presented that the soluble enzyme is not an artifact due to mitochondrial damage. 3. In contrast, pyruvate carboxylase in lactating rabbit mammary gland is confined to the mitochondrial fraction. 4. The final product of pyruvate carboxylase action in the mitochondrial and particle-free supernatant fractions of lactating rat mammary gland was shown to be citrate. 5. The effects of freeze-drying, ultrasonic treatment and freezing-and-thawing on the specific activity of mitochondrial pyruvate carboxylase were investigated.

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Year:  1969        PMID： 5812939      PMCID： PMC1187507          DOI： 10.1042/bj1110263

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  23 in total

1.  PYRUVATE CARBOXYLASE. II. PROPERTIES.

Authors:  D B KEECH; M F UTTER
Journal:  J Biol Chem       Date:  1963-08       Impact factor: 5.157

2.  PYUVATE-OXALOACETATE EXCHANGE REACTION IN BAKER'S YEAST.

Authors:  J GAILIUSIS; R W RINNE; C R BENEDICT
Journal:  Biochim Biophys Acta       Date:  1964-12-23

3.  Fatty acid biosynthesis. 3. Intracellular site of enzymes in lactating-rabbit mammary gland.

Authors:  S Smith; D J Easter; R Dils
Journal:  Biochim Biophys Acta       Date:  1966-12-07

4.  Enzymatic changes associated with the initiation and maintenance of lactation in the rat.

Authors:  R L Baldwin; L P Milligan
Journal:  J Biol Chem       Date:  1966-05-10       Impact factor: 5.157

5.  Paths of carbon in gluconeogenesis and lipogenesis. 3. The role and regulation of mitochondrial processes involved in supplying precursors of phosphoenolpyruvate.

Authors:  P Walter; V Paetkau; H A Lardy
Journal:  J Biol Chem       Date:  1966-06-10       Impact factor: 5.157

6.  Association of integrated metabolic pathways with membranes. I. Glycolytic enzymes of the red blood corpuscle and yeast.

Authors:  D E Green; E Murer; H O Hultin; S H Richardson; B Salmon; G P Brierley; H Baum
Journal:  Arch Biochem Biophys       Date:  1965-12       Impact factor: 4.013

7.  Adsorption of lactate dehydrogenase to the particulate fraction of homogenized skeletal muscle.

Authors:  H O Hultin; C Westort; J H Southard
Journal:  Nature       Date:  1966-08-20       Impact factor: 49.962

8.  The citrate cleavage pathway and lipogenesis in rat adipose tissue: replenishment of oxaloacetate.

Authors:  F J Ballard; R W Hanson
Journal:  J Lipid Res       Date:  1967-03       Impact factor: 5.922

9.  Citrate and the conversion of carbohydrate into fat. A comparison of citrate and acetate incorporation into fatty acids.

Authors:  A Spencer; L Corman; J M Lowenstein
Journal:  Biochem J       Date:  1964-11       Impact factor: 3.857

10.  Citrate and the conversion of carbohydrate into fat. Citrate cleavage in obesity and lactation.

Authors:  A F Spencer; J M Lowenstein
Journal:  Biochem J       Date:  1966-06       Impact factor: 3.857
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  7 in total

1.  The intracellular location of pyruvate carboxylase, citrate synthase and 3-hydroxyacyl-CoA dehydrogenase in lactating rat mammary gland.

Authors:  D J Taylor; B Crabtree; G H Smith
Journal:  Biochem J       Date:  1978-04-01       Impact factor: 3.857

2.  The intracellular localization of enzymes in white-adipose-tissue fat-cells and permeability properties of fat-cell mitochondria. Transfer of acetyl units and reducing power between mitochondria and cytoplasm.

Authors:  B R Martin; R M Denton
Journal:  Biochem J       Date:  1970-05       Impact factor: 3.857

3.  Immunochemical studies with soluble and mitochondrial pyruvate carboxylase activities from rat tissues.

Authors:  F J Ballard; R W Hanson; L Reshef
Journal:  Biochem J       Date:  1970-10       Impact factor: 3.857

4.  Enzymic changes in rabbit and rat mammary gland during the lactation cycle.

Authors:  B Gul; R Dils
Journal:  Biochem J       Date:  1969-04       Impact factor: 3.857

5.  Control of glucose metabolism in isolated acini of the lactating mammary gland of the rat. The ability of glycerol to mimic some of the effects of insulin.

Authors:  A M Robinson; D H Williamson
Journal:  Biochem J       Date:  1977-12-15       Impact factor: 3.857

6.  Lactating porcine mammary tissue catabolizes branched-chain amino acids for glutamine and aspartate synthesis.

Authors:  Peng Li; Darrell A Knabe; Sung Woo Kim; Christopher J Lynch; Susan M Hutson; Guoyao Wu
Journal:  J Nutr       Date:  2009-06-23       Impact factor: 4.798

7.  Validation-based model selection for 13C metabolic flux analysis with uncertain measurement errors.

Authors:  Nicolas Sundqvist; Nina Grankvist; Jeramie Watrous; Jain Mohit; Roland Nilsson; Gunnar Cedersund
Journal:  PLoS Comput Biol       Date:  2022-04-11       Impact factor: 4.779
  7 in total

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