Literature DB >> 5810049

Immune reactions in polysaccharide media. Polysaccharide-enhanced precipitin reactions with antigens of various sizes.

K Hellsing.   

Abstract

The influence of the size of the antigen in the polysaccharide-enhanced precipitin reaction was investigated. The experiments were carried out by addition of homologous antigens of different molecular sizes (the monomer, dimer and trimer of serum albumin and the monomer and dimer of immunoglobulin G) to the same preparation of antibody in the absence and presence of dextran. Dextran decreased the solubility of the immune complexes to a larger extent when the antigen size increased. This is in accord with the view that the polysaccharide effect is due to steric exclusion.

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Year:  1969        PMID: 5810049      PMCID: PMC1184807          DOI: 10.1042/bj1140145

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  8 in total

1.  Efficient trace-labelling of proteins with iodine.

Authors:  A S McFARLANE
Journal:  Nature       Date:  1958-07-05       Impact factor: 49.962

2.  [Inspissation of cerebrospinal fluid in preparation for paper electrophoresis; a simple and protective procedure].

Authors:  H J MIES
Journal:  Klin Wochenschr       Date:  1953-02-15

3.  Studies on circulating immune complexes. 3. Factors governing the ability of circulating complexes to localize in blood vessels.

Authors:  C G Cochrane; D Hawkins
Journal:  J Exp Med       Date:  1968-01-01       Impact factor: 14.307

4.  Immunochemical quantitation of antigens by single radial immunodiffusion.

Authors:  G Mancini; A O Carbonara; J F Heremans
Journal:  Immunochemistry       Date:  1965-09

5.  Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases.

Authors:  L M Siegel; K J Monty
Journal:  Biochim Biophys Acta       Date:  1966-02-07

6.  Immune reactions in polysaccharide media. Investigation on complex-formation between some polysaccharides, albumin and immunoglobulin G.

Authors:  K Hellsing
Journal:  Biochem J       Date:  1969-05       Impact factor: 3.857

7.  Immune reactions in polysaccharide media. The composition of the antigen-antibody complexes in the precipitin reaction.

Authors:  K Hellsing
Journal:  Biochem J       Date:  1969-08       Impact factor: 3.857

8.  Immune reactions in polysaccharide media. The effect of hyaluronate, chondroitin sulphate and chondroitin sulphate-protein complex on the precipitin reaction.

Authors:  K Hellsing
Journal:  Biochem J       Date:  1969-05       Impact factor: 3.857

  8 in total
  9 in total

1.  Distribution of free and antibody-bound peptide hormones in two-phase aqueous polymer systems.

Authors:  B Desbuquois; G D Aurbach
Journal:  Biochem J       Date:  1972-02       Impact factor: 3.857

2.  Protease and ribonuclease activities in bovine pituitary lobes.

Authors:  J C Pickup; D B Hope
Journal:  Biochem J       Date:  1971-06       Impact factor: 3.857

3.  Immune reactions in polysaccharide media. Experiments with specific antibodies of different affinities for serum albumin.

Authors:  K Hellsing
Journal:  Biochem J       Date:  1969-08       Impact factor: 3.857

4.  The exclusion of human serum albumin by human dermal collagenous fibres and within human dermis.

Authors:  J L Bert; J M Mathieson; R H Pearce
Journal:  Biochem J       Date:  1982-02-01       Impact factor: 3.857

5.  Improved streptococcal grouping antisera containing polyethylene glycol.

Authors:  J R George; H Ashworth; R R Facklam; W K Harrell; D F Palmer
Journal:  J Clin Microbiol       Date:  1981-10       Impact factor: 5.948

6.  Rapid screening for Staphylococcus aureus infection by measuring enterotoxin B.

Authors:  K K Hosotsubo; H Hosotsubo; M K Nishijima; M Nishimura; N Taenaka; I Yoshiya
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948

7.  Immune reactions in polysaccharide media. The composition of the antigen-antibody complexes in the precipitin reaction.

Authors:  K Hellsing
Journal:  Biochem J       Date:  1969-08       Impact factor: 3.857

8.  Electrophoretic and immunological characterization of rat neurophysin.

Authors:  A Norström; J Sjöstrand; B G Livett; L O Uttenthal; D B Hope
Journal:  Biochem J       Date:  1971-05       Impact factor: 3.857

9.  Solubility properties of IgG immune complexes. Comparison between the effects of low molecular weight solvents and polyethylene glycols.

Authors:  A S Johansen; J Steensgaard; C Jacobsen
Journal:  Immunology       Date:  1980-11       Impact factor: 7.397

  9 in total

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