| Literature DB >> 5807214 |
Abstract
1. A procedure for preparing crystalline 3-phosphoglycerate kinase from rabbit or pig skeletal muscle is presented. 2. The preparation phosphorylates up to 975mumoles of 3-phosphoglycerate/min./mg. at 30 degrees and is not contaminated with myokinase. 3. The enzyme has an estimated molecular weight of 36500+/-1000, and contains three residues each of tyrosine and tryptophan. 4. The preparation is suitable for use in the enzymic procedures for determining ATP, phosphocreatine and 3-phosphoglycerate.Entities:
Mesh:
Substances:
Year: 1969 PMID: 5807214 PMCID: PMC1184698 DOI: 10.1042/bj1130551
Source DB: PubMed Journal: Biochem J ISSN: 0264-6021 Impact factor: 3.857