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Literature DB >> 5803630

L-asparaginase production by Erwinia aroideae.

Abstract

Maximum yields of 1,250 IU (international unit)/g (dry weight of cells) of L-asparaginase were obtained in 8 hr from Erwinia aroideae NRRL B-138. Partial purification and concentration of the extracted L-asparaginase yielded a preparation with an activity of 275 IU/ml. Only one L-asparaginase was present as determined by electrophoresis, and the enzyme exhibited a pH optimum of 7.5 and a K(m) of 3 x 10(-3) M.

Entities: Species

Mesh：

Substances：
Asparaginase

Year: 1969 PMID： 5803630 PMCID： PMC377887 DOI： 10.1128/am.18.1.64-67.1969

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

4 in total

1. Fermentor for small-scale submerged fermentations.

Authors: R G DWORSCHACK; A A LAGODA; R W JACKSON
Journal: Appl Microbiol Date: 1954-07

Review 2. Antitumor activity and other biologic properties of L-asparaginase (NSC-109229)-a review.

Authors: R H Adamson; S Fabro
Journal: Cancer Chemother Rep Date: 1968-10

3. Inhibition of leukemias in man by L-asparaginase.

Authors: H F Oettgen; L J Old; E A Boyse; H A Campbell; F S Philips; B D Clarkson; L Tallal; R D Leeper; M K Schwartz; J H Kim
Journal: Cancer Res Date: 1967-12 Impact factor: 12.701

4. Evidence that the L-asparaginase of guinea pig serum is responsible for its antilymphoma effects. I. Properties of the L-asparaginase of guinea pig serum in relation to those of the antilymphoma substance.

Authors: J D BROOME
Journal: J Exp Med Date: 1963-07 Impact factor: 14.307

4 in total

17 in total

6. A comparison of L-asparaginase from Erwinia aroideae and from Escherichia coli: biochemical and biological properties.

Authors: J Staerk; O Zwisler; H Ronneberger
Journal: Experientia Date: 1971-03-15

7. L-asparaginase of Thermus thermophilus: purification, properties and identification of essential amino acids for its catalytic activity.

Authors: A A Pritsa; D A Kyriakidis
Journal: Mol Cell Biochem Date: 2001-01 Impact factor: 3.396

L-asparaginase production by Erwinia aroideae.

1. Fermentor for small-scale submerged fermentations.

Review 2. Antitumor activity and other biologic properties of L-asparaginase (NSC-109229)-a review.

3. Inhibition of leukemias in man by L-asparaginase.

4. Evidence that the L-asparaginase of guinea pig serum is responsible for its antilymphoma effects. I. Properties of the L-asparaginase of guinea pig serum in relation to those of the antilymphoma substance.

1. L-asparaginase of Tetrahymena pyriformis is associated with a kinase activity.

2. Fermentation kinetics and continuous process of L-asparaginase production.

3. Factors influencing L-asparaginase production by Erwinia aroideae.

4. Purification and properties of a membrane-bound L-asparaginase of Tetrahymena pyriformis.

5. Multi level statistical optimization of l-asparaginase from Bacillus subtilis VUVD001.

6. A comparison of L-asparaginase from Erwinia aroideae and from Escherichia coli: biochemical and biological properties.

7. L-asparaginase of Thermus thermophilus: purification, properties and identification of essential amino acids for its catalytic activity.

8. Purification, Characterization, and Effect of Thiol Compounds on Activity of the Erwinia carotovora L-Asparaginase.

9. L-Asparaginase from Proteus vulgaris.

10. Purification and properties of L-asparaginase from Serratia marcescens.