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Literature DB >> 5803537

Heterogeneity of presumably homogeneous protein preparations.

Abstract

Some highly purified glycolytic enzymes have been subjected to isoelectric focusing and found to contain a number of enzymatically active species. Crystalline aldolase A and glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle were resolved into five components, crystalline aldolase from yeast was resolved into three components, pyruvate kinase from rabbit muscle yielded four components, and yeast enolase was resolved into two components. Rabbit muscle lactate dehydrogenase (M(4)) gave one major peak of protein and enzymatic activity. The profiles of aldolase, glyceraldehyde-3-phosphate dehydrogenase, and yeast aldolases suggest random combinations of two closely related subunits into tetramers and dimers, respectively. The molecular heterogeneity of the other enzymes is not so easily related to subunit structure.

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Year: 1969 PMID： 5803537 DOI： 10.1126/science.165.3899.1260

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

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Cited

17 in total

1. The thin-layer isoelectric focusing of lactate dehydrogenase isoenzymes in rabbit lens parts and in intraocular tissues.

Authors: J Bours; H Neuhaus; O Hockwin
Journal: Albrecht Von Graefes Arch Klin Exp Ophthalmol Date: 1977-07-08

7. Phosphoglucomutase isozymes of red cells in Mus musculus: additional polymorphism at PGM-l compared by two electrophoretic systems.

Authors: G D Miner; H G Wolfe
Journal: Biochem Genet Date: 1972-12 Impact factor: 1.890

8. alpha-Amylase Isozymes in Gibberellic Acid-treated Barley Half-seeds.

Authors: Y Tanaka; T Akazawa
Journal: Plant Physiol Date: 1970-10 Impact factor: 8.340

9. Multiple forms of acidic endopeptidase from germinated barley.

Authors: W C Burger
Journal: Plant Physiol Date: 1973-06 Impact factor: 8.340

10. Isoelectric points of erabutoxins and monoacyl derivatives of erabutoxin b. Estimation of the pK values of amino groups in erabutoxins by using isoelectric-focusing data.

Authors: N Ui; C Takasaki; N Tamiya
Journal: Biochem J Date: 1982-05-01 Impact factor: 3.857

Heterogeneity of presumably homogeneous protein preparations.

1. The thin-layer isoelectric focusing of lactate dehydrogenase isoenzymes in rabbit lens parts and in intraocular tissues.

2. Relationship between in vivo degradative rates and isoelectric points of proteins.

3. Effect of NAD on flounder muscle glyceraldehyde 3-phosphate dehydrogenase.

4. 28 kDa adenosine-binding proteins of brain and other tissues.

Review 5. Current problems on the structure and classification of mammalian liver carboxylesterases (EC 3.1.1.1).

6. Deamidation in vivo of an asparagine residue of rabbit muscle aldolase.

7. Phosphoglucomutase isozymes of red cells in Mus musculus: additional polymorphism at PGM-l compared by two electrophoretic systems.

8. alpha-Amylase Isozymes in Gibberellic Acid-treated Barley Half-seeds.

9. Multiple forms of acidic endopeptidase from germinated barley.

10. Isoelectric points of erabutoxins and monoacyl derivatives of erabutoxin b. Estimation of the pK values of amino groups in erabutoxins by using isoelectric-focusing data.