Literature DB >> 579300

[Purification of the peptide hormone relaxin (author's transl)].

M Weiss, H Struck.   

Abstract

A simple procedure for the purification of the peptide hormone relaxin is described. It allows prepare with good yield highly purified relaxin of an activity of 700 GPU/mg protein. For the first time ion-exchange chromatography by Amberlite IRA-410 and subsequent gel filtration on sephadex G-25 fine were used as purifications steps.

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Year:  1977        PMID： 579300     DOI： 10.1007/bf00667393

Source DB:  PubMed          Journal:  Arch Gynakol        ISSN： 0003-9128


  10 in total

1.  [IMMUNOHISTOLOGICAL STUDIES ON THE LOCALIZATION OF RELAXIN IN THE HUMAN PLACENTA AND DECIDUA].

Authors:  F D DALLENBACH; G DALLENBACH-HELLWEG
Journal:  Virchows Arch Pathol Anat Physiol Klin Med       Date:  1964-02-27

2.  Bioassay of relaxin using a reference standard: a simple and reliable method utilizing direct measurement of interpubic ligament formation in mice.

Authors:  B G STEINETZ; V L BEACH; R L KROC; N R STASILLI; R E NUSSBAUM; P J NEMITH; R K DUN
Journal:  Endocrinology       Date:  1960-07       Impact factor: 4.736

3.  Purification of relaxin from sow ovaries.

Authors:  H COHEN; W KLEINBERG
Journal:  Arch Biochem Biophys       Date:  1960-11       Impact factor: 4.013

4.  Anaphylactic reaction to relaxin.

Authors:  R A LEVINE; R J KOSSMANN; B ROGOFF
Journal:  N Engl J Med       Date:  1960-10-06       Impact factor: 91.245

5.  Non-sterid ovarian hormones. II. An improved method for the preparation of relaxin.

Authors:  E H FRIEDEN; N W LAYMAN
Journal:  J Biol Chem       Date:  1957-12       Impact factor: 5.157

6.  The concentration of relaxin in the blood serum and other tissues of women during pregnancy.

Authors:  M X ZARROW; E G HOLMSTROM; H A SALHANICK
Journal:  J Clin Endocrinol Metab       Date:  1955-01       Impact factor: 5.958

7.  Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.

Authors:  R A REISFELD; U J LEWIS; D E WILLIAMS
Journal:  Nature       Date:  1962-07-21       Impact factor: 49.962

8.  Relaxin in the ovary of the domestic sow, Sus scrofa L.

Authors:  F L HISAW; M X ZARROW
Journal:  Proc Soc Exp Biol Med       Date:  1948-12

9.  Purification and characterization of porcine relaxin.

Authors:  C D Sherwood; E M O'Byrne
Journal:  Arch Biochem Biophys       Date:  1974-01       Impact factor: 4.013

10.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157
  10 in total

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