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Literature DB >> 5782447

Hormone storage granules in the beef anterior pituitary. I. Isolation, ultrastructure, and some biochemical properties.

Abstract

A method is described for isolation of relatively large quantities of large and small hormone storage granules from the beef adenohypophysis. The hormone storage granules are highly purified, as indicated by ultrastructural and biochemical criteria. The average size of large granules is 400 mmicro and of small granules is 220 mmicro. The large granules contain growth hormone and prolactin; the small granules contain high concentrations of follicle-stimulating, luteinizing, and thyroid-stimulating hormones. An alkaline protease with a pH optimum of 8.3 is associated with the small granule fraction.

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Year: 1969 PMID： 5782447 PMCID： PMC2107604 DOI： 10.1083/jcb.40.1.225

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

28 in total

1. THE FUNCTION OF THE GOLGI APPARATUS IN LACTATING CELLS OF THE BALB/CCRGL MOUSE. AN ELECTRON MICROSCOPIC AND AUTORADIOGRAPHIC STUDY.

Authors: S R WELLINGS; J R PHILP
Journal: Z Zellforsch Mikrosk Anat Date: 1964-01-31

2. Effect of diethylstilbestrol on the quantity and intracellular distribution of pituitary proteinase activity.

Authors: R K MEYER; K H CLIFTON
Journal: Arch Biochem Biophys Date: 1956-05 Impact factor: 4.013

3. Fractionation of pituitary homogenates by differential centrifugation. I. Association of hormonal activity with subcellular fractions.

Authors: J B MELCHIOR; D M ZIEGLER
Journal: J Biol Chem Date: 1956-10 Impact factor: 5.157

7. Studies on hormones from the anterior pituitary gland. I. Identification and isolation of growth hormone and prolactin from the "granular" fraction of bovine pituitary.

Authors: H G Kwa; E M van der Bent; C A Feltkamp; P Rümke; H Bloemendal
Journal: Biochim Biophys Acta Date: 1965-12-16

Review 8. The cells of the adenohypophysis and their functional significance.

Authors: M Herlant
Journal: Int Rev Cytol Date: 1964

9. Cytoplasmic granule formation in myelocytes. An electron microscope radioautographic study on the mechanism of formation of cytoplasmic granules in rabbit heterophilic myelocytes.

Authors: M E Fedorko; J G Hirsch
Journal: J Cell Biol Date: 1966-05 Impact factor: 10.539

Hormone storage granules in the beef anterior pituitary. I. Isolation, ultrastructure, and some biochemical properties.

1. THE FUNCTION OF THE GOLGI APPARATUS IN LACTATING CELLS OF THE BALB/CCRGL MOUSE. AN ELECTRON MICROSCOPIC AND AUTORADIOGRAPHIC STUDY.

2. Effect of diethylstilbestrol on the quantity and intracellular distribution of pituitary proteinase activity.

3. Fractionation of pituitary homogenates by differential centrifugation. I. Association of hormonal activity with subcellular fractions.

4. Electron microscopic studies of the anterior pituitary gland of castrate rats.

5. Assay of the follicle stimulating hormone based on the augmentation with human chorionic gonadotropin.

6. [Separation of the activity of the anterior lobe of the pituitary by the method of differential centrifugation].

7. Studies on hormones from the anterior pituitary gland. I. Identification and isolation of growth hormone and prolactin from the "granular" fraction of bovine pituitary.

Review 8. The cells of the adenohypophysis and their functional significance.

9. Cytoplasmic granule formation in myelocytes. An electron microscope radioautographic study on the mechanism of formation of cytoplasmic granules in rabbit heterophilic myelocytes.

10. Isolation and biochemical study of secretory granules from rat pituitary glands.

1. Protease and ribonuclease activities in bovine pituitary lobes.

2. Isolation and biological properties of secretory granules from rat anterior pituitary glands.