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Literature DB >> 5781586

Hydrogenase measurement with photochemically reduced methyl viologen.

Abstract

Methyl viologen was reduced photochemically in the presence of proflavine and ethylenediaminetetraacetic acid. The reduced methyl viologen was oxidized by hydrogenase from Vibrio succinogenes. H(2) and oxidized methyl viologen were the products. Hydrogenase activity was determined by spectrophotometric measurement of the disappearance of reduced methyl viologen at 600 nm. The extinction coefficient of reduced methyl viologen was determined and is 8.25 mm(-1) x cm(-1) at 600 nm. Optimal conditions for assaying V. succinogenes hydrogenase were developed. Extracts of Escherichia coli and Desulfovibrio desulfuricans catalyzed reduced methyl viologen oxidation in the assay system, whereas Azotobacter vinelandii extracts were inactive.

Entities: Chemical Species

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Year: 1969 PMID： 5781586 PMCID： PMC249902 DOI： 10.1128/jb.98.1.51-55.1969

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

8 in total

1. Formic dehydrogenase and the hydrogenlyase enzyme complex in coli-aerogenes bacteria.

Authors: H D PECK; H GEST
Journal: J Bacteriol Date: 1957-06 Impact factor: 3.490

2. A study of the hydrogenlyase reaction with systems derived from normal and anaerogenic coli-aerogenes bacteria.

Authors: H GEST; H D PECK
Journal: J Bacteriol Date: 1955-09 Impact factor: 3.490

3. A new procedure for assay of bacterial hydrogenases.

Authors: H D PECK; H GEST
Journal: J Bacteriol Date: 1956-01 Impact factor: 3.490

4. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

5. Solubilization and reconstitution of a particulate hydrogenase from Vibrio succinogenes.

Authors: A J Aspen; M J Wolin
Journal: J Biol Chem Date: 1966-09-25 Impact factor: 5.157

6. Molecular H2 and the PN2 function of azotobacter.

Authors: G W Strandberg; P W Wilson
Journal: Proc Natl Acad Sci U S A Date: 1967-10 Impact factor: 11.205

7. Lysis of Vibrio succinogenes by ethylenediamine-tetraacetic acid or lysozyme.

Authors: M J Wolin
Journal: J Bacteriol Date: 1966-05 Impact factor: 3.490

8. Maintenance of the rumen microbial population in continuous culture.

Authors: W H RUFENER; W O NELSON; M J WOLIN
Journal: Appl Microbiol Date: 1963-05

8 in total

17 in total

1. Altered Electron Flow in Continuous Cultures of Clostridium acetobutylicum Induced by Viologen Dyes.

Authors: G Rao; R Mutharasan
Journal: Appl Environ Microbiol Date: 1987-06 Impact factor: 4.792

2. Structure-based alteration of substrate specificity and catalytic activity of sulfite oxidase from sulfite oxidation to nitrate reduction.

Authors: James A Qiu; Heather L Wilson; K V Rajagopalan
Journal: Biochemistry Date: 2012-01-30 Impact factor: 3.162

8. Partial purification of ferredoxin from Ruminococcus albus and its role in pyruvate metabolism and reduction of nicotinamide adenine dinucleotide by H2.

Authors: T L Glass; M P Bryant; M J Wolin
Journal: J Bacteriol Date: 1977-08 Impact factor: 3.490

9. Hydrogenase activity of mineral-associated and suspended populations of Desulfovibrio desulfuricans Essex 6.

Authors: C L Reardon; T S Magnuson; E S Boyd; W D Leavitt; D W Reed; G G Geesey
Journal: Microb Ecol Date: 2013-11-06 Impact factor: 4.552

10. Ferredoxin- and nicotinamide adenine dinucleotide-dependent H 2 production from ethanol and formate in extracts of S organism isolated from "Methanobacillus omelianskii".

Authors: C A Reddy; M P Bryant; M J Wolin
Journal: J Bacteriol Date: 1972-04 Impact factor: 3.490