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Literature DB >> 5774762

Vaccinia neutralization test using a radioisotope assay for virus-induced enzyme activity.

Abstract

Vaccinia virus induction of a metabolic activity in host cell cultures forms the basis of a new assay for neutralizing antibodies. A direct relationship between the amount of vaccinia virus infecting cell cultures and the induced incorporation of tritium-labeled thymidine into the acid-insoluble fraction of the cells provided an indicator system. A liquid scintillation spectrometer was used to determine radioactivity associated with cell materials, and it provided a method for partial automation of an immunological procedure. Reproducibility of the method was satisfactory, and agreement with conventional vaccinia serum neutralization tests was demonstrated.

Entities: Chemical Disease Species

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Year: 1969 PMID： 5774762 PMCID： PMC377609 DOI： 10.1128/am.17.1.41-47.1969

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

12 in total

3. Assay of neutralizing antibody against variola virus by the degree of focus reduction on HeLa cell cultures and its application to revaccination with smallpox vaccines of various potencies.

Authors: T Kitamura; N Shinjo
Journal: Bull World Health Organ Date: 1972 Impact factor: 9.408

3 in total

Vaccinia neutralization test using a radioisotope assay for virus-induced enzyme activity.

1. Amino acid metabolism in mammalian cell cultures.

2. DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.

3. ANTIBODY RESPONSE TO SMALLPOX VACCINATION AS EXPRESSED BY PLAQUE REDUCTION ON HELA CELLS.

4. Induction of thymidine kinase by vaccinia-infected mouse fibroblasts.

5. Acquisition of thymidine kinase activity by herpes simplex-infected mouse fibroblast cells.

6. The titration of vaccinial neutralizing antibody on chorio-allantoic membranes.

7. Titration of relative neutralizing potency of vaccinia immune serum in tube cultures of monkey kidney cells.

8. Protein measurement with the Folin phenol reagent.

9. Micro radiometric method for study of acid-insoluble materials in monolayer cell cultures.

10. A simplified plaque technique for titration of pox virus and its antibodies in chick embryo cells.

1. Micro radiometric method for study of acid-insoluble materials in monolayer cell cultures.

2. Kinetics of the vaccinia virus plaque neutralization test.

3. Assay of neutralizing antibody against variola virus by the degree of focus reduction on HeLa cell cultures and its application to revaccination with smallpox vaccines of various potencies.