Water soluble benzodiazepines with agonistic and antagonistic actions on GABA-induced inhibition in cultured hypothalamus.

The interaction of two benzodiazepine compounds, flurazepam (a sedative and RO 5-3663 (a convulsant), with amino acid depressions of spontaneous neuronal activity in hypothalamic tissue cultures has been examined. Flurazepam selectively potentiated, GABA-induced inhibition, while RO 5-3663 selectively reduced GABA-induced inhibtion. These results are interpreted as supporting the presence of two types of benzodiazepine binding sites in the brain which would modulate endogenous GABA-mediated inhibition and behavior.