Literature DB >> 5724

An assay of ribonuclease H, endoribonucleases, and phosphatases.

J G Starvianopoulos, E Chargaff.   

Abstract

An assay for ribonuclease H (EC 3.1.4.34) is described which permits the estimation of the number and the type of cleavages produced by the enzyme when acting on the ribo moiety of a DNA-RNA hybrid substrate. With six different homopolymer hybrids tested, the number of enzymically induced breaks varied widely. The method is applicable to other endoribonucleases, phosphodiesterases, and phosphatases.

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Year:  1976        PMID： 5724      PMCID： PMC430336          DOI： 10.1073/pnas.73.5.1556

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  4 in total

1.  NUCLEOTIDE SEQUENCE ANALYSIS OF POLYRIBONUCLEOTIDES BY MEANS OF PERIODATE OXIDATION FOLLOWED BY CLEAVAGE WITH AN AMINE.

Authors:  H C NEU; L A HEPPEL
Journal:  J Biol Chem       Date:  1964-09       Impact factor: 5.157

2.  A hydrolytic procedure for ribonucleosides and its possible application to the sequential degradation of RNA.

Authors:  C T YU; P C ZAMECNIK
Journal:  Biochim Biophys Acta       Date:  1960-12-04

3.  Purification and properties of ribonuclease H of calf thymus.

Authors:  J G Stavrianopoulos; E Chargaff
Journal:  Proc Natl Acad Sci U S A       Date:  1973-07       Impact factor: 11.205

4.  Ribonuclease H of calf thymus: substrate specificity, activation, inhibition.

Authors:  J G Stavrianopoulos; A Gambino-Giuffrida; E Chargaff
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205
  4 in total
  1 in total

1.  Simplified method for purification of ribonuclease H from calf thymus.

Authors:  J G Stavrianopoulos; E Chargaff
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205
  1 in total

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