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Literature DB >> 571603

Further characterisation of a nucleic acid binding protein.

Abstract

A glycoprotein which binds to nucleic acids has now been purified from Ustilago maydis until free from detectable deoxyribonuclease activity. It binds to a variety of substrates and in doing so, makes them soluble in dilute trichloroacetic acid. Physical studies suggest that it forms a variety of aggregates under low ionic strength, but at high ionic strength the monomer consists of a single polypeptide chain. Preliminary experiments have detected this novel binding activity in bacterial, fungal and mammalian cells.

Entities: Chemical Species

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Year: 1979 PMID： 571603 PMCID： PMC327743 DOI： 10.1093/nar/6.3.931

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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References

19 in total

1. The isolation and properties of a DNA-unwinding protein from Ustilago maydis.

Authors: G R Banks; A Spanos
Journal: J Mol Biol Date: 1975-03-25 Impact factor: 5.469

2. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.

Authors: C C RICHARDSON; C L SCHILDKRAUT; H V APOSHIAN; A KORNBERG
Journal: J Biol Chem Date: 1964-01 Impact factor: 5.157

10. DNA "melting" proteins. II. Effects of bacteriophage T4 gene 32-protein binding on the conformation and stability of nucleic acid structures.

Authors: D E Jensen; R C Kelly; P H von Hippel
Journal: J Biol Chem Date: 1976-11-25 Impact factor: 5.157

Further characterisation of a nucleic acid binding protein.

1. The isolation and properties of a DNA-unwinding protein from Ustilago maydis.

2. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI.

3. Enzymatic synthesis of deoxyribonucleic acid. VII. Synthesis of a polymer of deoxyadenylate and deoxythymidylate.

4. A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

5. Genetic characterization of rec-1, a mutant of Ustilago maydis defective in repair and recombination.

6. Solubility and dialysis limits of DNA oligonucleotides.

7. The binding of sodium dodecyl sulphate to various proteins.

8. A protein from Ustilago which forms an acid-soluble complex with deoxyribonucleic acid.

9. A DNA polymerase from Ustilago maydis. 1. Purification and properties of the polymerase activity.

10. DNA "melting" proteins. II. Effects of bacteriophage T4 gene 32-protein binding on the conformation and stability of nucleic acid structures.