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Literature DB >> 5707

Effect of excision of the Y-base on the interaction of tRNAPhe (yeast) with phenylalanyl-tRNA synthetase (yeast).

Abstract

The interaction between tRNAPhe (yeast), from which the Y-base has been removed by acid treatment, and phenylalanyl-tRNA synthetase (yeast) has been investigated by fluorescence competition titrations and sedimentation velocity runs. The binding parameters are given under various ionic conditions. The tRNAPhe-Y still can occupy the specific binding sites on the enzyme. Compared to unmodified tRNAPhe, the binding constant is lowered by more than one order of magnitude. It can be concluded that the Y-base is not necessary for specific recognition of tRNAPhe by the cognate synthetase, it rather may represent a point of attachment for the synthetase.

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Year: 1976 PMID： 5707 PMCID： PMC342929 DOI： 10.1093/nar/3.3.631

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

2. Biochemical and structural characterization of the flavodoxin-like domain of the Schizosaccharomyces japonicus putative tRNA ^Phe 4-demethylwyosine synthase Tyw1 in complex with FMN.

Authors: Ljiljana Sjekloća; Adrian R Ferré-D'Amaré
Journal: MicroPubl Biol Date: 2022-06-08

2 in total

Effect of excision of the Y-base on the interaction of tRNAPhe (yeast) with phenylalanyl-tRNA synthetase (yeast).

1. NMR study on the methyl and methylene proton resonances of tRNA Phe yeast.

2. Effect of the removal of the Y base on the conformation of yeast tRNA.

3. Function of Y in codon-anticodon interaction of tRNA Phe .

4. Removal of Y-37 from tRNA phe yeast alters oligomer binding to two loops.

5. The general structure of transfer RNA molecules.

6. Further studies on amino acid acceptance and physical properties of tRNA-phe-yeast.

7. A simple procedure for the purification of yeast phenylalanine transfer RNA.

8. [Purification and properties of phenylalanyl-tRNA synthetase from baker's yeast].

9. Equivalent and non-equivalent binding sites for tRNA on aminoacyl-tRNA synthetases.

10. Kinetic studies on the interaction of seryl-tRNA synthetase with tRNA(Ser) and ser-tRNA(ser) from yeast.

1. Mechanism of tRNA-synthetase recognition: role of terminal A.

2. Biochemical and structural characterization of the flavodoxin-like domain of the Schizosaccharomyces japonicus putative tRNA ^Phe 4-demethylwyosine synthase Tyw1 in complex with FMN.