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Literature DB >> 5690994

DNA organization in the mature sperm of several orthoptera by the method of polarized fluorescence microscopy.

Abstract

Mature sperm of Acheta domesticus, Acheta assimilis, Nemobius fasciatus, Nemobius confusus, Melanoplus femur-rubrum, Romalea microptera, Scudderia curvicauda, and Ceuthophilus nigricans were examined for DNA configuration by means of polarized fluorescence microscopy. In all cases, the results suggest that the DNA lies in unsupercoiled array, predominantly parallel to the elongate sperm head axes. Detailed calculations of the factors influencing the degree of polarization of the fluorescent emission from supercoiled DNA are contained in an appendix.

Entities: Disease Species

Mesh：

Substances：
DNA

Year: 1968 PMID： 5690994 PMCID： PMC2107478 DOI： 10.1083/jcb.38.2.426

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

9 in total

4 in total

DNA organization in the mature sperm of several orthoptera by the method of polarized fluorescence microscopy.

1. Sleep deprivation, age, and exhaustion time in the rat.

2. Crystallinity in sperm heads: molecular structure of nucleoprotein in vivo.

3. Intrinsic Birefringence of Multiple-Coiled DNA, Theory and Applications.

4. Supercoiling of polyoma virus DNA measured by its interaction with ethidium bromide.

5. Changes in deoxyribonucleoprotein during spermiogenesis in the bull.

6. Spectral analysis of the binding of acridine orange to polytene chromosomes.

7. Organization of DNA in dipteran polytene chromosomes as indicated by polarized fluorescence microscopy.

8. Thermal depolarization of fluorescence from polytene chromosomes stained with acridine orange.

9. THE ARRANGEMENT OF CHROMOSOMES IN THE MATURE SPERM OF THE GRASSHOPPER.

1. The interaction of acridine dyes with the densely packed DNA of bacteriophage.

2. The orientation of DNA within 80-Angstrom chromatin fibers.

3. Birefringence of spermatozoa. I. Birefringence melting of squid, bull, and human sperm nucleoprotein.

4. [Linear dichroitic studies of DNA organization in sperm heads of Locusta migratoria L].