Literature DB >> 5679361

Nuclease activity in cell-free amino acid-incorporating systems from chicken liver.

J G Siler, M Fried.   

Abstract

1. Comparative studies of the polyuridylic acid-directed phenylalanine-incorporating activity of cell-free systems derived from rat and chicken livers demonstrated markedly lower activity in the chicken liver system. 2. The chicken liver cell sap contained the factor(s) responsible for this lower activity. Ribosomes from chicken and rat performed equally well in the presence of rat liver cell sap. Chicken liver cell sap, when mixed with rat liver cell sap, caused an inhibition of incorporation of phenylalanine into acid-insoluble material. 3. Though ribosomal preparations and cell sap from both rat and chicken liver degraded polyuridylic acid to some extent, the chicken liver cell sap contained the largest amount of activity. 4. Rat liver cell sap inhibited the nuclease activities of ribosomal preparations, but no such nuclease inhibition could be demonstrated with chicken liver cell sap.

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Year:  1968        PMID: 5679361      PMCID: PMC1186773          DOI: 10.1042/bj1090185

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  9 in total

1.  INTERACTION OF RIBOSOMES AND SYNTHETIC POLYRIBONUCLEOTIDES.

Authors:  M TAKANAMI; T OKAMOTO
Journal:  J Mol Biol       Date:  1963-10       Impact factor: 5.469

2.  CHARACTERIZATION OF RIBOSOMAL AGGREGATES ISOLATED FROM LIVER.

Authors:  R R HOWELL; J N LOEB; G M TOMKINS
Journal:  Proc Natl Acad Sci U S A       Date:  1964-11       Impact factor: 11.205

3.  Ribonuclease. IX. Further studies on ribonuclease inhibitor.

Authors:  J S ROTH
Journal:  Biochim Biophys Acta       Date:  1962-12-31

4.  Ribonuclease. VII. Partial purification and characterization of a ribonuclease inhibitor in rat liver supernatant fraction.

Authors:  J S ROTH
Journal:  J Biol Chem       Date:  1958-04       Impact factor: 5.157

5.  Ribonuclease. V. Studies on the properties and distribution of ribonuclease inhibitor in the rat.

Authors:  J S ROTH
Journal:  Biochim Biophys Acta       Date:  1956-07

6.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

7.  The inhibition of rat liver polyribosome breakdown in the presence of liver supernatant.

Authors:  G R Lawford; P Langford; H Schachter
Journal:  J Biol Chem       Date:  1966-04-25       Impact factor: 5.157

8.  The mechanism of protein synthesis in the developing chick embryo. The incorporation of free amino acids.

Authors:  N H Carey
Journal:  Biochem J       Date:  1964-05       Impact factor: 3.857

9.  Amino acid incorporation by microsomal fractions from the liver of the domestic fowl.

Authors:  P J Heald; D Pohlman
Journal:  Biochim Biophys Acta       Date:  1966-08-17
  9 in total
  2 in total

1.  Inhibitory effects of pH5 enzyme from non-lactating bovine mammary gland on various stages of protein synthesis in the rat liver amino acid-incorporating system.

Authors:  M D Herrington; A O Hawtrey
Journal:  Biochem J       Date:  1969-12       Impact factor: 3.857

2.  Polymorphism in fowl serum albumin. VI. Changes in in vitro protein synthesizing activity in developing embryonic fowl liver.

Authors:  M H Chu; H M Jernigan; M A Iacona; M Fried
Journal:  Mol Cell Biochem       Date:  1979-04-02       Impact factor: 3.396

  2 in total

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