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Literature DB >> 5669842

The preparation and properties of pyruvate kinase attached to porous sheets, and the operation of a two-enzyme continuous-feed reactor.

Abstract

1. The covalent attachment of pyruvate kinase to filter-paper disks and some kinetic properties of the resultant enzymically active porous sheets are described. 2. A two-enzyme reactor was constructed by using water-insoluble sheets of lactate dehydrogenease in conjunction with the above-mentioned sheets possessing pyruvate kinase activity.

Entities: Chemical

Mesh：

Substances：

Year: 1968 PMID： 5669842 PMCID： PMC1186762 DOI： 10.1042/bj1090137

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

11 in total

1. A WATER-INSOLUBLE POLYANIONIC DERIVATIVE OF TRYPSIN. II. EFFECT OF THE POLYELECTROLYTE CARRIER ON THE KINETIC BEHAVIOR OF THE BOUND TRYPSIN.

Authors: L GOLDSTEIN; Y LEVIN; E KATCHALSKI
Journal: Biochemistry Date: 1964-12 Impact factor: 3.162

2. The correlation of reaction kinetics and substrate binding with the mechanism of pyruvate kinase.

Authors: A M REYNARD; L F HASS; D D JACOBSEN; P D BOYER
Journal: J Biol Chem Date: 1961-08 Impact factor: 5.157

3. Physical properties of crystalline fluorokinase.

Authors: R C WARNER
Journal: Arch Biochem Biophys Date: 1958-12 Impact factor: 4.013

4. Some changes in the reactivity of enzymes resulting from their chemical attachment to water-insoluble derivatives of cellulose.

Authors: W E Hornby; M D Lilly
Journal: Biochem J Date: 1968-05 Impact factor: 3.857

The preparation and properties of pyruvate kinase attached to porous sheets, and the operation of a two-enzyme continuous-feed reactor.

1. A WATER-INSOLUBLE POLYANIONIC DERIVATIVE OF TRYPSIN. II. EFFECT OF THE POLYELECTROLYTE CARRIER ON THE KINETIC BEHAVIOR OF THE BOUND TRYPSIN.

2. The correlation of reaction kinetics and substrate binding with the mechanism of pyruvate kinase.

3. Physical properties of crystalline fluorokinase.

4. Some changes in the reactivity of enzymes resulting from their chemical attachment to water-insoluble derivatives of cellulose.

5. Preparation and use of porous sheets with enzyme action.

Review 6. Water-insoluble derivatives of enzymes, antigens, and antibodies.

7. The effect of univalent cation salts on the stability and on certain physical properties of pyruvate kinase.

8. Coupling of enzymes to cellulose using chloro-s-triazines.

9. The preparation and kinetics of lactate dehydrogenase attached to water-insoluble particles and sheets.

10. The kinetics of carboxymethylcellulose--ficin in packed beds.

1. Comparative studies on soluble and immobilized rabbit muscle pyruvate kinase.