Literature DB >> 5667526

Centrifugation of mammalian cells on gradients: a new rotor.

T G Pretlow, C W Boone.   

Abstract

A short-arm rotor increases separation of viable mammalian cells, from mixtures, by low-speed centrifugation; continuous Ficoll density gradients in tissue-culture media are used. We describe the theory and experimental demonstration of the superior separation achieved with this new rotor.

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Year:  1968        PMID： 5667526     DOI： 10.1126/science.161.3844.911

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  8 in total

1.  A method for the separation of lymphocytes and plasma cells from the human palatine tonsil using sedimentation in an isokinetic gradient of Ficoll in tissue culture medium.

Authors:  J K Willson; D E Luberoff; A Pitts; T C Pretlow
Journal:  Immunology       Date:  1975-01       Impact factor: 7.397

2.  Separation of spleen colony forming units (CEU-S) from mouse bone marrow cells using velocity sedimentation in an isokinetic gradient of Ficoll in tissue culture medium.

Authors:  T G Pretlow; E E Williams; M L Davis; J G Zettergren
Journal:  Am J Pathol       Date:  1973-08       Impact factor: 4.307

3.  Separation of beating cardiac myocytes from suspensions of heart cells.

Authors:  T G Pretlow; M R Glick; W J Reddy
Journal:  Am J Pathol       Date:  1972-05       Impact factor: 4.307

4.  Separation of mast cells in successive stages of differentiation using programmed gradient sedimentation.

Authors:  T G Pretlow; I M Cassady
Journal:  Am J Pathol       Date:  1970-12       Impact factor: 4.307

5.  Separation of acinar cells from lactating mammary gland using velocity sedimentation in an isokinetic gradient of Ficoll in tissue culture medium.

Authors:  T G Pretlow; S R Dow; T M Murad; J Jones
Journal:  Am J Pathol       Date:  1974-07       Impact factor: 4.307

6.  Separation of lymphocytes and macrophages from suspensions of guinea pig peritonitis exudate cells using programmed gradient sedimentation.

Authors:  T G Pretlow; M E Pichichero; L Hyams
Journal:  Am J Pathol       Date:  1971-05       Impact factor: 4.307

7.  Velocity sedimentation of organelles at low centrifugal force in an isokinetic gradient.

Authors:  T G Pretlow; J I Kreisberg; W D Fine; G A Zieman; M G Brattain; T P Pretlow
Journal:  Biochem J       Date:  1978-07-15       Impact factor: 3.857

8.  Separation of ascites myeloma cells, lymphocytes and macrophages by zonal centrifugation on an isokinetic gradient.

Authors:  M J Stewart; T G Pretlow; R Hiramoto
Journal:  Am J Pathol       Date:  1972-07       Impact factor: 4.307
  8 in total

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