Literature DB >> 5664209

Mass preparation of nuclei from the larval salivary glands of Drosophila hydei.

J B Boyd, H D Berendes, H Boyd.   

Abstract

A method has been developed for isolating gram quantities of salivary glands from late third instar larvae of Drosophila hydei. The isolated glands have a normal appearance and incorporate RNA and DNA precursors normally. Nuclei can be isolated from these glands in 90% yield with the use of detergents. These nuclei contain morphologically normal giant polytene chromosomes.

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Year:  1968        PMID： 5664209      PMCID： PMC2107489          DOI： 10.1083/jcb.38.2.369

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  10 in total

1.  EXPERIMENTAL PUFFS IN SALIVARY GLAND CHROMOSOMES OF DROSOPHILA HYDEI.

Authors:  H D BERENDES; T K HOLT
Journal:  Chromosoma       Date:  1965-01-30       Impact factor: 4.316

2.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  A system in vitro for the synthesis of RNA and protein by isolated salivary glands and by nuclei from Chironomus larvae.

Authors:  H Ristow; S Arends
Journal:  Biochim Biophys Acta       Date:  1968-03-18

5.  Ordered replication of DNA in polytene chromosomes of Drosophila melanogaster.

Authors:  W Plaut; D Nash; T Fanning
Journal:  J Mol Biol       Date:  1966-03       Impact factor: 5.469

6.  Use of a single tissue extract to determine cellular protein and nucleic acid concentrations and rate of amino acid incorporation.

Authors:  R W Wannemacher; W L Banks; W H Wunner
Journal:  Anal Biochem       Date:  1965-05       Impact factor: 3.365

7.  Electron microscopic studies of detergent-treated HeLa cell nuclei.

Authors:  E Holtzman; I Smith; S Penman
Journal:  J Mol Biol       Date:  1966-05       Impact factor: 5.469

8.  Proteins in nucleocytoplasmic interactions. I. The fundamental characteristics of the rapidly migrating proteins and the slow turnover proteins of the Amoeba proteus nucleus.

Authors:  L Goldstein; D M Prescott
Journal:  J Cell Biol       Date:  1967-06       Impact factor: 10.539

9.  Isolation of metaphase chromosomes from HeLa cells.

Authors:  J A Huberman; G Attardi
Journal:  J Cell Biol       Date:  1966-10       Impact factor: 10.539

10.  The preparative isolation of imaginal discs from larvae of Drosophila melanogaster.

Authors:  J W Fristrom; H K Mitchell
Journal:  J Cell Biol       Date:  1965-11       Impact factor: 10.539
  10 in total
  18 in total

1.  The proteins of polytene chromosomes of Drosophila hydei.

Authors:  S C Elgin; J B Boyd
Journal:  Chromosoma       Date:  1975       Impact factor: 4.316

2.  Induced transcription-dependent synthesis of mitochondrial reduced nicotinamide-adenine dinucleotide dehydrogenase in Drosophila.

Authors:  J F Koninkx
Journal:  Biochem J       Date:  1975-10       Impact factor: 3.857

3.  Protein synthesis in salivary glands of Drosophila hydei after experimental gene induction.

Authors:  J F Koninkx
Journal:  Biochem J       Date:  1976-09-15       Impact factor: 3.857

4.  The effect of changes in the respiratory metabolism upon genome activity in Drosophila. I. The induction of gene activity.

Authors:  H J Leenders; H D Berendes
Journal:  Chromosoma       Date:  1972       Impact factor: 4.316

5.  Deoxyribonucleic acid synthesis in isolated polytene nuclei of Drosophila hydei.

Authors:  J B Boyd; J M Presley
Journal:  Biochem Genet       Date:  1973-08       Impact factor: 1.890

6.  The effect of changes in the respiratory metabolism upon genome activity. A correlation between induced gene activity and an increase in activity of a respiratory enzyme.

Authors:  H J Leenders; P J Beckers
Journal:  J Cell Biol       Date:  1972-11       Impact factor: 10.539

7.  [Problems of the chromosome structure of Mesostoma].

Authors:  H G Keyl; F Göltenboth
Journal:  Chromosoma       Date:  1972       Impact factor: 4.316

8.  Deoxyribonucleases of the organs of Drosophila hydei at the onset of metamorphosis.

Authors:  J B Boyd; H Boyd
Journal:  Biochem Genet       Date:  1970-08       Impact factor: 1.890

9.  Polytene chromosome structure at the submicroscopic level. II. Length distribution of DNA molecules from polytene chromosomes of Drosophila melanogaster and D. hydei.

Authors:  J Derksen; H D Berendes
Journal:  Chromosoma       Date:  1970       Impact factor: 4.316

10.  Chemical and conformational changes in chromosome regions being actively transcribed.

Authors:  M Pagés; C Alonso
Journal:  Nucleic Acids Res       Date:  1978-02       Impact factor: 16.971
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