Literature DB >> 5663301

Bioluminescence: pH activity profiles of related luciferase fractions.

N Krieger, J W Hastings.   

Abstract

The 27, 000g supernatant from crude extracts of the dinoflagellate Gonyaulax can be fractionated into two components having luciferase activity that differ both in molecular weight (35, 000 and 150, 000) and in pH activity profile. The smaller component has activity over a broad range from pH 5 to 9, while the larger one is active only in the acid region. This clarifies the previous ambiguity in the literature regarding the optimum pH for the assay of luciferase.

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Year:  1968        PMID： 5663301     DOI： 10.1126/science.161.3841.586

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  11 in total

1.  Voltage-gated proton channel in a dinoflagellate.

Authors:  Susan M E Smith; Deri Morgan; Boris Musset; Vladimir V Cherny; Allen R Place; J Woodland Hastings; Thomas E Decoursey
Journal:  Proc Natl Acad Sci U S A       Date:  2011-10-17       Impact factor: 11.205

2.  Profile of J. Woodland Hastings.

Authors:  Tinsley H Davis
Journal:  Proc Natl Acad Sci U S A       Date:  2007-01-10       Impact factor: 11.205

Review 3.  Philosophy of voltage-gated proton channels.

Authors:  Thomas E DeCoursey; Jonathan Hosler
Journal:  J R Soc Interface       Date:  2013-12-18       Impact factor: 4.118

Review 4.  Voltage-gated proton channels: molecular biology, physiology, and pathophysiology of the H(V) family.

Authors:  Thomas E DeCoursey
Journal:  Physiol Rev       Date:  2013-04       Impact factor: 37.312

5.  Bioluminescence: mechanism and mode of control of scintillon activity.

Authors:  M Fogel; J W Hastings
Journal:  Proc Natl Acad Sci U S A       Date:  1972-03       Impact factor: 11.205

6.  Solubilization of molecular elements of scintillons: bioluminescent particles from dinoflagellates.

Authors:  J P Henry; J W Hastings
Journal:  Mol Cell Biochem       Date:  1974-04-15       Impact factor: 3.396

Review 7.  The Voltage-Gated Proton Channel: A Riddle, Wrapped in a Mystery, inside an Enigma.

Authors:  Thomas E DeCoursey
Journal:  Biochemistry       Date:  2015-05-20       Impact factor: 3.162

Review 8.  Voltage-gated proton channels.

Authors:  Thomas E Decoursey
Journal:  Compr Physiol       Date:  2012-04       Impact factor: 9.090

9.  Two different domains of the luciferase gene in the heterotrophic dinoflagellate Noctiluca scintillans occur as two separate genes in photosynthetic species.

Authors:  Liyun Liu; J Woodland Hastings
Journal:  Proc Natl Acad Sci U S A       Date:  2006-11-27       Impact factor: 11.205

10.  Identification of a vacuolar proton channel that triggers the bioluminescent flash in dinoflagellates.

Authors:  Juan D Rodriguez; Saddef Haq; Tsvetan Bachvaroff; Kristine F Nowak; Scott J Nowak; Deri Morgan; Vladimir V Cherny; Maredith M Sapp; Steven Bernstein; Andrew Bolt; Thomas E DeCoursey; Allen R Place; Susan M E Smith
Journal:  PLoS One       Date:  2017-02-08       Impact factor: 3.240
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