Literature DB >> 5647523

Differential chromatographic bioassay for the gentamicin complex.

G H Wagman, E M Oden, M J Weinstein.   

Abstract

A differential paper chromatographic assay for the components of the gentamicin complex, designated as C(1), C(1a), and C(2), is described. The gentamicins are separated on paper strips with the lower phase of a solvent mixture consisting of chloroform, methanol, and 17% ammonium hydroxide (2: 1: 1, v/v). After chromatographic separation, the paper strips are plated against Staphylococcus aureus ATCC 6538P, and the zones of activity are quantified against known standards.

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Year:  1968        PMID： 5647523      PMCID： PMC547482          DOI： 10.1128/am.16.4.624-627.1968

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  6 in total

1.  GENTAMICIN, A NEW ANTIBIOTIC COMPLEX FROM MICROMONOSPORA.

Authors:  M J WEINSTEIN; G M LUEDEMANN; E M ODEN; G H WAGMAN; J P ROSSELET; J A MARQUEZ; C T CONIGLIO; W CHARNEY; H L HERZOG; J BLACK
Journal:  J Med Chem       Date:  1963-07       Impact factor: 7.446

2.  ISOLATION, PURIFICATION, AND CHARACTERIZATION OF GENTAMICIN.

Authors:  J P ROSSELET; J MARQUEZ; E MESECK; A MURAWSKI; A HAMDAN; C JOYNER; R SCHMIDT; D MIGLIORE; H L HERZOG
Journal:  Antimicrob Agents Chemother (Bethesda)       Date:  1963

3.  The chemistry of kanamycin.

Authors:  M J CRON; O B FARDIG; D L JOHNSON; F M PALERMITI; H SCHMITZ; I R HOOPER
Journal:  Ann N Y Acad Sci       Date:  1958-09-30       Impact factor: 5.691

4.  A PAPER CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE ESTIMATION OF PENICILLIN ENTITIES.

Authors:  R G Kluener
Journal:  J Bacteriol       Date:  1949-01       Impact factor: 3.490

5.  Quantitative determination of the components of neomycin by paper chromatography.

Authors:  M K Majumdar; S K Majumdar
Journal:  Anal Chem       Date:  1967-02       Impact factor: 6.986

6.  Biological activity of the antibiotic components of the gentamicin complex.

Authors:  M J Weinstein; G H Wagman; E M Oden; J A Marquez
Journal:  J Bacteriol       Date:  1967-09       Impact factor: 3.490
  6 in total
  1 in total

1.  The bifunctional enzyme, GenB4, catalyzes the last step of gentamicin 3',4'-di-deoxygenation via reduction and transamination activities.

Authors:  Xiaotang Chen; Hui Zhang; Shaotong Zhou; Mingjun Bi; Shizhou Qi; Huiyuan Gao; Xianpu Ni; Huanzhang Xia
Journal:  Microb Cell Fact       Date:  2020-03-10       Impact factor: 5.328
  1 in total

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