Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Heat-stable chemically defined medium for growth of animal cells in suspension.

Literature DB >> 5636473

Heat-stable chemically defined medium for growth of animal cells in suspension.

Abstract

Procedures are described for preparation of a chemically defined medium, capable of being autoclaved, for the cultivation of animal cells in suspension. Yields of 34.1 x 10(5), 33.8 x 10(5), and 47.1 x 10(5) cells per ml were recorded for cat kidney, HeLa, and mouse fibroblast (L) cells after 6, 10, and 10 days of incubation, respectively.

Entities: Species

Mesh：

Substances：
Culture Media

Year: 1968 PMID： 5636473 PMCID： PMC547308 DOI： 10.1128/am.16.1.53-55.1968

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

4 in total

1. A chemically defined medium for growth of animal cells in suspension.

Authors: S C NAGLE; H R TRIBBLE; R E ANDERSON; N D GARY
Journal: Proc Soc Exp Biol Med Date: 1963-02

2. A growth-stimulating factor for an epithelial cell line in a reduced serum medium.

Authors: T P SERGEANT; S SMITH
Journal: Science Date: 1960-02-26 Impact factor: 47.728

3. The submerged culture of mammalian cells; the spinner culture.

Authors: W F McLIMANS; E V DAVIS; F L GLOVER; G W RAKE
Journal: J Immunol Date: 1957-11 Impact factor: 5.422

4. Detergents in membrane filters.

Authors: R D Cahn
Journal: Science Date: 1967-01-13 Impact factor: 47.728

4 in total

5 in total

1. Growth of Chlamydia psittaci strain meningopneumonitis in mouse L cells cultivated in a defined medium in spinner cultures.

Authors: S J Morrison; H M Jenkin
Journal: In Vitro Date: 1972 Sep-Oct

Heat-stable chemically defined medium for growth of animal cells in suspension.

1. A chemically defined medium for growth of animal cells in suspension.

2. A growth-stimulating factor for an epithelial cell line in a reduced serum medium.

3. The submerged culture of mammalian cells; the spinner culture.

4. Detergents in membrane filters.

1. Growth of Chlamydia psittaci strain meningopneumonitis in mouse L cells cultivated in a defined medium in spinner cultures.

2. Epstein-Barr virus from P3HR-1 cells grown in chemically defined medium.

3. Growth of mouse L cells in shaken culture and mengovirus plaque formation on L cells suspended in agar.

4. Improved growth of mammalian and insect cells in media containing increased levels of choline.

5. Establishment of Namalva cell lines which grow continuously in glutamine-free medium.