Literature DB >> 5636329

Antigen binding to cells: determination by enzymic fluorogenic group hydrolysis.

E E Sercarz, F Modabber.   

Abstract

A sensitive method for detecting cells containing antibody to beta-galactosidase has been devised. The enzyme attached to the cells containing antibody can hydrolyze a fluorogenic substrate and yield fluorescent products which are measured microphotofluorometrically. This method of detecting a few molecules of antibody is applicable to other enzyme antigen systems.

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Year:  1968        PMID： 5636329     DOI： 10.1126/science.159.3817.884

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  6 in total

1.  Affinity of antigen for white cells and its relation to the induction of antibody formation.

Authors:  D Sulitzeanu
Journal:  Bacteriol Rev       Date:  1968-12

2.  Antibody-mediated activation of a defective beta-D-galactosidase extracted from an Escherichia coli mutant.

Authors:  M B Rotman; F Celada
Journal:  Proc Natl Acad Sci U S A       Date:  1968-06       Impact factor: 11.205

3.  Binding of antigen-enzyme complex by antigen-binding cells as an approach for immunogdiagnosis.

Authors:  F Z Modabber; G M Bahr
Journal:  Infect Immun       Date:  1979-01       Impact factor: 3.441

4.  Regulation of the immune system by synthetic polynucleotides. V. Effect on cell-associated immunoglobulin receptors and immunological memory.

Authors:  R D Stout; A G Johnson
Journal:  J Exp Med       Date:  1972-01       Impact factor: 14.307

5.  Studies on membrane-bound receptors for antigen. Preparation of populations of receptor-depleted lymphocytes.

Authors:  R D Stout; A H Coons
Journal:  J Exp Med       Date:  1973-10-01       Impact factor: 14.307

6.  Cell interactions in the primary immune response in vitro: a requirement for specific cell clusters.

Authors:  D E Mosier
Journal:  J Exp Med       Date:  1969-02-01       Impact factor: 14.307
  6 in total

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