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Literature DB >> 56256

Cytochemical studies on RNP complexes produced by puff 2-48BC in Drosophila hydei: uranyl acetate and phosphotungstic acid staining.

Abstract

Differential staining of the core and RNP particles of RNP complexes in puff 2--48 BC in salivary gland chromosomes of Drosophila hydei was achieved with aqueous uranyl acetate (UA) at low pH, with UA in acetone, with phosphotungstic acid (PTA) in organic solvents, and with aqueous PTA at pH 5 And 6. A comparison of the results of UA and PTA staining under various conditions indicate that the proteins in the core region and in the RNP particles connected to it differ with respect to their amino-acid composition (arginine and lysine residues).--The staining mechanism of PTA and UA is discussed.

Entities: Chemical Species

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Year: 1976 PMID： 56256 DOI： 10.1007/bf00288327

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

28 in total

2. A comparative ultrastructural study of 'glue' production and secretion of the salivary glands in different species of theDrosophila melanogaster group.

Authors: G N Thomopoulos; C D Kastritsis
Journal: Wilehm Roux Arch Dev Biol Date: 1979-12

3. Heat-shock puff 93 D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function.

Authors: A Dangli; C Grond; P Kloetzel; E K Bautz
Journal: EMBO J Date: 1983 Impact factor: 11.598

3 in total

Cytochemical studies on RNP complexes produced by puff 2-48BC in Drosophila hydei: uranyl acetate and phosphotungstic acid staining.

1.

2. CYTOCHEMICAL STUDIES ON NUCLEAR FINE STRUCTURE.

3. Nuclear differentiation and functional morphology of chromosomes.

4. Some properties of RNA:protein complexes from the nucleus of eukaryotic cells.

5. On phosphotungstic acid staining. IV. Selectivity of the staining reaction.

6. Cytochemical studies on chromosome ultrastructure.

7. RNA transport from nucleus to cytoplasm in Chironomus salivary glands.

8. Improvements in epoxy resin embedding methods.

9. Electron stains. I. Chemical studies on the interaction of DNA with uranyl salts.

10. The reactivity and staining of tissue proteins with phosphotungstic acid.

1. Dynamics of hnRNPs and omega speckles in normal and heat shocked live cell nuclei of Drosophila melanogaster.

2. A comparative ultrastructural study of 'glue' production and secretion of the salivary glands in different species of theDrosophila melanogaster group.

3. Heat-shock puff 93 D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function.