Literature DB >> 562236

Selective release of chromosomal proteins during limited DNAase 1 digestion of avian erythrocyte chromatin.

G Vidali, L C Boffa, V G Allfrey.   

Abstract

Duck erythrocyte chromatin has been treated with DNAase 1 under conditions that are known to digest selectively the structural genes coding for globin mRNAs. This limited digestion releases specific sets of nonhistone chromosomal proteins that are not preferentially released during limited digestion with micrococcal nuclease, which does not selectively attack the globin sequences. Analysis of nucleosome monomer and multimer peaks separated on sucrose gradients after limited digestion with micrococcal nuclease shows that the proteins which are released by DNAase 1 digestion remain associated with the chromatin subunits and can be removed by extraction in 0.5 M NaCl. These proteins are tentatively identified as members of the high mobility group (HMG) proteins (originally described by Goodwin, Sanders and Johns, 1973) in terms of their extractability, electrophoretic characteristics and amino acid composition.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 562236     DOI： 10.1016/0092-8674(77)90117-9

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  35 in total

1.  Blotting Index of Dissimilarity: use to study immunological relatedness of plant and animal High Mobility Group (HMG) chromosomal proteins.

Authors:  S Spiker; K M Everett
Journal:  Plant Mol Biol       Date:  1987-09       Impact factor: 4.076

2.  Purification and characterization of a high-mobility-group-like DNA-binding protein that stimulates rRNA synthesis in vitro.

Authors:  H F Yang-Yen; L I Rothblum
Journal:  Mol Cell Biol       Date:  1988-08       Impact factor: 4.272

3.  Comparison of non histone proteins selectively associated with nucleosomes with proteins released during limited DNase digestions.

Authors:  N Defer; M Crepin; C Terrioux; J Kruh; F Gros
Journal:  Nucleic Acids Res       Date:  1979-03       Impact factor: 16.971

4.  DNA-binding properties of a nonhistone chromosomal protein from lymphocytes.

Authors:  H Blüthmann
Journal:  Mol Cell Biochem       Date:  1978-05-31       Impact factor: 3.396

5.  Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.

Authors:  S Weisbrod; H Weintraub
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

6.  Hemoglobin switching in sheep: only the gamma gene is in the active conformation in fetal liver but all the beta and gamma genes are in the active conformation in bone marrow.

Authors:  N S Young; E J Benz; J A Kantor; P Kretschmer; A W Nienhuis
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

7.  HMG proteins (1 + 2) form beaded structures when complexed with closed circular DNA.

Authors:  D J Mathis; A Kindelis; C Spadafora
Journal:  Nucleic Acids Res       Date:  1980-06-25       Impact factor: 16.971

8.  Comparative analysis of proteins selectively released from cell nuclei of different eukaryotic species under conditions of mild hydrolysis by nucleases.

Authors:  B N Afanasjev; A A Karavanov; V V Chestkov
Journal:  Mol Biol Rep       Date:  1980-07-31       Impact factor: 2.316

9.  High mobility group proteins 1 and 2 are present in simian virus 40 provirions, but not in virions.

Authors:  F La Bella; M Romani; C Vesco; G Vidali
Journal:  Nucleic Acids Res       Date:  1981-01-10       Impact factor: 16.971

10.  Selective release of HMG nonhistone proteins during DNase digestion of Tetrahymena chromatin at different stages of the cell cycle.

Authors:  K Hamana; M Zama
Journal:  Nucleic Acids Res       Date:  1980-11-25       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.