Literature DB >> 561788

Homogeneous cell populations from rabbit kidney cortex. Proximal, distal tubule, and renin-active cell isolated by free-flow electrophoresis.

H G Heidrich, M E Dew.   

Abstract

A single-cell suspension has been prepared from rabbit kidney cortex by using a Ca-binding medium and gentle mechanical forces. The suspension was subjected to carrier-free electrophoresis, and several cell fractions were obtained. Proximal and distal tubule cell populations could be identified by their morphology. Renin-containing cells were located by means of radioimmunoassay. The morphology of the cells and their vitality (uridine incorporation) are discussed.

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Year:  1977        PMID: 561788      PMCID: PMC2110089          DOI: 10.1083/jcb.74.3.780

Source DB:  PubMed          Journal:  J Cell Biol        ISSN: 0021-9525            Impact factor:   10.539


  17 in total

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Authors:  S T JACOB; P M BHARGAVA
Journal:  Exp Cell Res       Date:  1962-09       Impact factor: 3.905

2.  Preparative free-flow electrophoresis for the isolation of membrane, organelle and cell fractions from rabbit kidney cortex.

Authors:  H G Heidrich; M E Dew
Journal:  Curr Probl Clin Biochem       Date:  1976

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Authors:  P Drochmans; J C Wanson; R Mosselmans
Journal:  J Cell Biol       Date:  1975-07       Impact factor: 10.539

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Authors:  B Baggio; S Favoro; A Antonello; A Zen; F Zen; A Borsatti
Journal:  Ital J Biochem       Date:  1975 May-Aug

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Authors:  H Bojar; K Balzer; F Boeminghaus; W Staib
Journal:  Z Klin Chem Klin Biochem       Date:  1975-01

6.  Sites of enzyme activity along the nephron.

Authors:  U Schmidt; W G Guder
Journal:  Kidney Int       Date:  1976-03       Impact factor: 10.612

7.  Criteria of viability of isolated liver cells.

Authors:  H Baur; S Kasperek; E Pfaff
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1975-06

8.  Isolation of renal tubules.

Authors:  G Rorive; A Kleinzeller
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

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Authors:  B Thimmappayya; R R Reddy; P M Bhargava
Journal:  Exp Cell Res       Date:  1970-12       Impact factor: 3.905

10.  Application of a radioimmunoassay for angiotensin I to the physiologic measurements of plasma renin activity in normal human subjects.

Authors:  E Haber; T Koerner; L B Page; B Kliman; A Purnode
Journal:  J Clin Endocrinol Metab       Date:  1969-10       Impact factor: 5.958

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  10 in total

Review 1.  [Study of kidney function using isolated cells].

Authors:  R K Kinne; C Grupp; R W Grunewald
Journal:  Klin Wochenschr       Date:  1990-02-15

2.  Primary monolayer culture of liver parenchymal cells and kidney cortical tubules as a useful new model for biochemical pharmacology and experimental toxicology. Studies in vitro on hepatic membrane transport, induction of liver enzymes, and adaptive changes in renal cortical enzymes.

Authors:  P Bellemann
Journal:  Arch Toxicol       Date:  1980-03       Impact factor: 5.153

3.  Isolation of cells from rabbit renal proximal tubules by using a hyperosmolar intracellular-like solution.

Authors:  C N Nagineni; P J Leveille; D B Lee; N Yanagawa
Journal:  Biochem J       Date:  1984-10-15       Impact factor: 3.857

4.  An in vitro test for measuring cytotoxicity of mercuric chloride to human kidney epithelial cells by specific enzyme release.

Authors:  D Merlet; M Mellado; J P Merlet; J Cambar
Journal:  Cytotechnology       Date:  1988-07       Impact factor: 2.058

5.  Primary culture of mammalian nephron epithelia: requirements for cell outgrowth and proliferation from defined explanted nephron segments.

Authors:  M Horster
Journal:  Pflugers Arch       Date:  1979-11       Impact factor: 3.657

6.  Sodium-chloride transport in the thick ascending limb of Henle's loop. Oxygen consumption studies in isolated cells.

Authors:  J Eveloff; E Bayerdörffer; P Silva; R Kinne
Journal:  Pflugers Arch       Date:  1981-03       Impact factor: 3.657

7.  Separation of renal medullary cells: isolation of cells from the thick ascending limb of Henle's loop.

Authors:  J Eveloff; W Haase; R Kinne
Journal:  J Cell Biol       Date:  1980-12       Impact factor: 10.539

8.  Cortical cell populations from rabbit kidney isolated by free-flow electrophoresis: characterization by measurement of hormone-sensitive adenylate cyclase.

Authors:  A Vandewalle; B Köpfer-Hobelsberger; H G Heidrich
Journal:  J Cell Biol       Date:  1982-02       Impact factor: 10.539

9.  Sodium-alanine cotransport in renal proximal tubule cells investigated by whole-cell current recording.

Authors:  J Hoyer; H Gögelein
Journal:  J Gen Physiol       Date:  1991-05       Impact factor: 4.086

10.  The MDCK epithelial cell line expresses a cell surface antigen of the kidney distal tubule.

Authors:  D A Herzlinger; T G Easton; G K Ojakian
Journal:  J Cell Biol       Date:  1982-05       Impact factor: 10.539

  10 in total

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