Literature DB >> 56173

The radioimmunoassay of steroid glucuronides. The oestrogen C-3 glucuronides as haptens.

P Samarajeewa, A E Kellie.   

Abstract

Antisera were prepared against three related oestrogen ring-A glucuronides, oestrone 3-glucuronide, oestradiol 3-glucuronide and oestriol 3-glucuronide. The corresponding 6,7-3H-labelled conjugates were synthesized as radioligands and the cross-reactions of the antisera against ring-A oestrogen glucuronides and other steroid conjugates were examined. The specificity of the antiserum against oestriol 3-glucuronide was compared with that raised against oestriol 16alpha-glucuronide, and the measurement of the former conjugate in late-pregnancy urine is discussed.

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Year:  1975        PMID： 56173      PMCID： PMC1172367          DOI： 10.1042/bj1510369

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  Steroid-protein conjugates. II. Preparation and characterization of conjugates of bovine serum albumin with progesterone, deoxycorticosterone, and estrone.

Authors:  B F ERLANGER; F BOREK; S M BEISER; S LIEBERMAN
Journal:  J Biol Chem       Date:  1959-05       Impact factor: 5.157

2.  Steroid-protein conjugates. I. Preparation and characterization of conjugates of bovine serum albumin with testosterone and with cortisone.

Authors:  B F ERLANGER; F BOREK; S M BEISER; S LIEBERMAN
Journal:  J Biol Chem       Date:  1957-10       Impact factor: 5.157

3.  Comparative studies on hydrolysis of conjugated estrogens in human pregnancy urine.

Authors:  R F STRAW; P A KATZMAN; E A DOISY
Journal:  Endocrinology       Date:  1955-07       Impact factor: 4.736

4.  Radioimmunoassay of steroids the role of the "bridge" linking the steroid hapten to the protein carrier.

Authors:  K Hoffmann; P Samarafeewa; E R Smith; A E Kellie
Journal:  J Steroid Biochem       Date:  1975-02       Impact factor: 4.292

5.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

6.  The excretion of oestrogen conjugates in late pregnancy urine.

Authors:  J Ahmed; A E Kellie
Journal:  J Steroid Biochem       Date:  1972-01       Impact factor: 4.292

7.  Antigenic complexes of steroid hormones formed by coupling to protein through position 7: preparation from 4 -3-oxosteroids and characterization of antibodies to testosterone and androstenedione.

Authors:  A Weinstein; H R Lindner; A Friedlander; S Bauminger
Journal:  Steroids       Date:  1972-12       Impact factor: 2.668

8.  Selective deuterium labeling of steroidal estrogens.

Authors:  J H Block; C Djerassi
Journal:  Steroids       Date:  1973-11       Impact factor: 2.668

9.  Steroid glucuronoside-BSA complexes as antigens: the radioimmunoassay of steroid conjugates.

Authors:  A E Kellie; V K Samuel; W J Riley; D M Robertson
Journal:  J Steroid Biochem       Date:  1972-04       Impact factor: 4.292

10.  A study of the colorimetric estimation of oestradiol-17 beta, oestradiol-17 alpha, oestrone, oestriol and 16-epioestriol by the Kober reaction.

Authors:  W NOCKE
Journal:  Biochem J       Date:  1961-03       Impact factor: 3.857
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  1 in total

1.  The direct radioimmunoassay of oestrogen glucuronides in human female urine.

Authors:  T S Baker; K M Jennison; A E Kellie
Journal:  Biochem J       Date:  1979-02-01       Impact factor: 3.857
  1 in total

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