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Literature DB >> 560353

Uracil phosphoribosyl transferase activity of mycoplasma and infected cell cultures.

C W Long, R DelGiudice, R S Gardella, M Hatanaka.

Abstract

Human H.Ep-2 and mouse 3T6 cells infected by Mycoplasma hyorhinis showed an increase in [3H]uracil uptake and a more than 20-fold increase in the activity of uracil phosphoribosyltransferase (UraPRT). Uninfected cell cultures gave background levels of this enzyme activity. A survey of 16 strains of mycoplasma showed 13 to possess UraPRT activity. Rabbit kidney cells (RK13) were infected with eight different strains of four mycoplasma species known to be common cell culture contaminants. Seven of the eight cell cultures showed elevated UraPRT activities four days after infection. This enzyme activity may be of value in monitoring cell cultures for mycoplasma and aid in classification.

Entities: Chemical Disease Species

Mesh：

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Year: 1977 PMID： 560353 DOI： 10.1007/bf02615103

Source DB: PubMed Journal: In Vitro ISSN： 0073-5655

15 in total

1. STUDIES ON THE BIOSYNTHESIS OF 5-RIBOSYLURACIL 5'-MONOPHOSPHATE IN TETRAHYMENA PYRIFORMIS.

Authors: R L HEINRIKSON; E GOLDWASSER
Journal: J Biol Chem Date: 1964-04 Impact factor: 5.157

2. Conversion of uracil and orotate to uridine 5'-phosphate by enzymes in lactobacilli.

Authors: I CRAWFORD; A KORNBERG; E S SIMMS
Journal: J Biol Chem Date: 1957-06 Impact factor: 5.157

3. The synthesis of 5-fluorouridine 5'-phosphate by a pyrimidine phosphoribosyltransferase of mammalian origin. I. Some properties of the enzyme from P1534J mouse leukemic cells.

Authors: P Reyes
Journal: Biochemistry Date: 1969-05 Impact factor: 3.162

4. Absence of demonstrable linkage of human genes for enzymes of the purine and pyrimidine salvage pathways in human-mouse somatic cell hybrids.

Authors: C Long; T Chan; V Levytska; T Kusano; H Green
Journal: Biochem Genet Date: 1973-07 Impact factor: 1.890

5. Adenine formation from adenosine by mycoplasmas: adenosine phosphorylase activity.

Authors: M Hatanaka; R Del Giudice; C Long
Journal: Proc Natl Acad Sci U S A Date: 1975-04 Impact factor: 11.205

6. 3-RIBOSYLPURINES. I. SYNTHESIS OF (3-RIBOSYLURIC ACID) 5'-PHOSPHATE AND (3-RIBOSYLXANTHINE) 5'-PHOSPHATE BY A PYRIMIDINE RIBONUCLEOTIDE PYROPHOSPHORYLASE OF BEEF ERYTHROCYTES.

Authors: D HATFIELD; J B WYNGAARDEN
Journal: J Biol Chem Date: 1964-08 Impact factor: 5.157

Uracil phosphoribosyl transferase activity of mycoplasma and infected cell cultures.

1. STUDIES ON THE BIOSYNTHESIS OF 5-RIBOSYLURACIL 5'-MONOPHOSPHATE IN TETRAHYMENA PYRIFORMIS.

2. Conversion of uracil and orotate to uridine 5'-phosphate by enzymes in lactobacilli.

3. The synthesis of 5-fluorouridine 5'-phosphate by a pyrimidine phosphoribosyltransferase of mammalian origin. I. Some properties of the enzyme from P1534J mouse leukemic cells.

4. Absence of demonstrable linkage of human genes for enzymes of the purine and pyrimidine salvage pathways in human-mouse somatic cell hybrids.

5. Adenine formation from adenosine by mycoplasmas: adenosine phosphorylase activity.

6. 3-RIBOSYLPURINES. I. SYNTHESIS OF (3-RIBOSYLURIC ACID) 5'-PHOSPHATE AND (3-RIBOSYLXANTHINE) 5'-PHOSPHATE BY A PYRIMIDINE RIBONUCLEOTIDE PYROPHOSPHORYLASE OF BEEF ERYTHROCYTES.

7. Macromolecular synthesis and thymineless death in Mycoplasma laidlawii B.

8. Nucleoside incorporation into strain L cells: inhibition by pleuropneumonia-like organisms.

9. Alterations in the characteristics of sugar uptake by mouse cells transformed by murine sarcoma viruses.

10. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.

1. Uracil phosphoribosyltransferase from Acholeplasma laidlawii: partial purification and kinetic properties.

2. An autoradiographic method of detecting A. laidlawii and M. hyorhinis in cell cultures.

3. Differences in incorporation of nucleic acid bases and nucleosides by various Mycoplasma and Acholeplasma species.

4. Analysis of multiple isoenzyme expression among twenty-two species of Mycoplasma and Acholeplasma.