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Literature DB >> 5584011

Quantitiative nucleic acid changes during phytohaemagglutinin-induced lymphocyte transformation in vitro. Dependence of the response on phytohaemagglutinin-serum rati.

Abstract

1. Pig and human blood lymphocytes have been grown in culture without replenishment of medium, and stimulated to transform by phytohaemagglutinin. Quantitative nucleic acid changes during this process have been used as an index of transformation. 2. On the first day, cells attach to glass; then they detach and continue transforming. 3. The degree of transformation is dependent on the phytohaemagglutinin/serum ratio, and is independent of cell concentration within the range 0.5x10(6)-2.0x10(6) cells/ml. 4. At low phytohaemagglutinin/serum ratios there is no response; at high phytohaemagglutinin/serum ratios, inhibition appears after the cells have been cultured for a day.

Entities: Species

Mesh：

Substances：
Antibodies
RNA
DNA

Year: 1967 PMID： 5584011 PMCID： PMC1198363 DOI： 10.1042/bj1050679

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

38 in total

8. Continuous cinematography of human lymphocytes cultured with a phytohaemagglutinin including observations on cell division and interphase.

Authors: W H Marshall; K B Roberts
Journal: Q J Exp Physiol Cogn Med Sci Date: 1965-10

9. Induction of ribonucleic acid synthesis in human leucocytes by phytohemagglutinin.

Authors: G C Mueller; M L Mahieu
Journal: Biochim Biophys Acta Date: 1966-01-18

10. Phytohaemagglutinin-induced cytotoxic action of unsensitized immunologically competent cells on allogeneic and xenogeneic tissue culture cells.

Authors: G Holm; P Perlmann
Journal: Nature Date: 1965-08-21 Impact factor: 49.962

12 in total

4. Serum factors affecting the incorporation of (3H)thymidine by lymphocytes stimulated by antigen. I. Serum concentration.

Authors: D R Forsdyke
Journal: Immunology Date: 1973-10 Impact factor: 7.397

5. Inhibition of PHA-stimulated lymphocyte transformation by plasma from patients with advanced alcoholic cirrhosis.

Authors: C C Hsu; C M Leevy
Journal: Clin Exp Immunol Date: 1971-05 Impact factor: 4.330

6. Turnover of mammalian phospholipids. Rates of turnover and metabolic heterogeneity in cultured human lymphocytes and in tissues of healthy, starved and vitamin A-deficient rats.

Authors: C A Pasternak; B Friedrichs
Journal: Biochem J Date: 1970-09 Impact factor: 3.857

7. Studies on the incorporation of [5-3H] uridine during activation and transformation of lymphocytes induced by phytohaemagglutinin. Dependence on the incorporation rate on uridine concentration at certain critical concentrations.

Authors: D R Forsdyke
Journal: Biochem J Date: 1968-03 Impact factor: 3.857

8. Application of the isotope-dilution principle to the analysis of factors affecting the incorporation of (3H) uridine and (3H) cytidine into cultured lymphocytes. Evaluation of pools in serum and culture media.

Authors: D R Forsdyke
Journal: Biochem J Date: 1971-12 Impact factor: 3.857

9. Serum factors affecting the incorporation of (3H)uridine by lymphocytes stimulated by concanavalin A. Studies of the role of complement.

Authors: P Milthorp; D R Forsdyke
Journal: Biochem J Date: 1973-04 Impact factor: 3.857

10. Early effects of phytohaemagglutinin on glucose metabolism of normal human lymphocytes.

Authors: C J Hedeskov
Journal: Biochem J Date: 1968-11 Impact factor: 3.857

Quantitiative nucleic acid changes during phytohaemagglutinin-induced lymphocyte transformation in vitro. Dependence of the response on phytohaemagglutinin-serum rati.

1. EDITORIAL. (BERTEL VON BONSDORFF).

Review 2. TISSUE CULTURE STUDIES OF THE HUMAN LYMPHOCYTE.

3. SEPARATION OF VIABLE LYMPHOCYTES FROM HUMAN BLOOD.

4. THE ACTION OF STREPTOLYSIN S ON PERIPHERAL LYMPHOCYTES OF NORMAL SUBJECTS AND PATIENTS WITH ACUTE RHEUMATIC FEVER.

5. Effect of phytohaemagglutinin on human serum and cell proteins.

6. Studies in histochemistry. I. Determination of nucleic acids in microgram amounts of tissue.

7. Studies of lymphocytes and their derivative cells in vitro. I. Biochemical constituents.