Literature DB >> 558275

A method for enucleating oocytes of Xenopus laevis.

C C Ford, J B Gurdon.   

Abstract

A method is described for the enucleation and complete healing of Xenopus oocytes so that the enucleated oocytes withstand multiple injections and culture for several days. The oocytes are defolliculated and enucleated manually and allowed to heal in a potassium phosphate buffer. Oocytes enucleated in this way support RNA synthesis by injected HeLa-cell nuclei 3 days later. This method is valuable in providing a low-background system in which transcription of injected nuclei can be studied.

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Year:  1977        PMID： 558275

Source DB:  PubMed          Journal:  J Embryol Exp Morphol        ISSN： 0022-0752


  14 in total

1.  Nuclear processing of the 3'-terminal nucleotides of pre-U1 RNA in Xenopus laevis oocytes.

Authors:  H Yang; M L Moss; E Lund; J E Dahlberg
Journal:  Mol Cell Biol       Date:  1992-04       Impact factor: 4.272

2.  H-ras(val12) induces cytoplasmic but not nuclear events of the cell cycle in small Xenopus oocytes.

Authors:  A D Johnson; R J Cork; M A Williams; K R Robinson; L D Smith
Journal:  Cell Regul       Date:  1990-06

3.  Essential role of germinal vesicle material in the meiotic cell cycle of Xenopus oocytes.

Authors:  J Iwashita; Y Hayano; N Sagata
Journal:  Proc Natl Acad Sci U S A       Date:  1998-04-14       Impact factor: 11.205

4.  The control of cell volume: frog eggs face an extreme challenge.

Authors:  P T Macklem; S Kelly
Journal:  Trans Am Clin Climatol Assoc       Date:  1990

5.  Intron-less RNA injected into the nucleus of Xenopus oocytes accesses a regulated translation control pathway.

Authors:  M Braddock; M Muckenthaler; M R White; A M Thorburn; J Sommerville; A J Kingsman; S M Kingsman
Journal:  Nucleic Acids Res       Date:  1994-12-11       Impact factor: 16.971

6.  Subcellular distribution of ribosomal proteins S6 and eL12. Analysis by autoradiography and immunofluorescence of sections from oocytes of Xenopus laevis.

Authors:  D Darmer; H Kalthoff; D Richter
Journal:  Cell Tissue Res       Date:  1984       Impact factor: 5.249

7.  Nuclear routing networks span between nuclear pore complexes and genomic DNA to guide nucleoplasmic trafficking of biomolecules.

Authors:  Marek Malecki; Bianca Malecki
Journal:  J Fertili In Vitro       Date:  2012-10-19

8.  Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.

Authors:  P E Auron; A C Webb; L J Rosenwasser; S F Mucci; A Rich; S M Wolff; C A Dinarello
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205

9.  Choline acetyltransferase and acetylcholine in Xenopus oocytes injected with mRNA from the electric lobe of Torpedo.

Authors:  C B Gundersen; D J Jenden; R Miledi
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

10.  Transplantation of insect giant chromosome nuclei into amphibian oocytes.

Authors:  U Grossbach; I Streichhan
Journal:  Chromosoma       Date:  1981       Impact factor: 4.316
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